Universal Methylated Mouse DNA Standard

The Universal Methylated Mouse DNA Standard is designed for use as control to assess the efficiency of bisulfite-mediated conversion of DNA in combination with the EZ DNA Methylation™, EZ DNA Methylation- Gold™, or EZ DNA Methylation-Direct™ kits. The control DNA has been enzymatically modified in vitro with CpG Methylase, resulting in methylation at all cytosines in the dinucleotide sequence 5"²...CpG...3"². The methylated cytosines remain unconverted following bisulfite treatment, whereas non-methylated cytosines are converted into uracils and detected as thymines following PCR. Each primer set has been specifically designed to amplify a fragment of the supplied DNA following bisulfite treatment.