5-hmC Enrichment/detection

ENRICHMENT
The protein, JBP-1, binds glucosyl-5-hmC with high affinity and is the key component in the Quest 5-hmC™ Enrichment Kit for selective enrichment of 5-hmC DNA from genomic DNA preparations. Enriched DNA is ideal for Next-Gen sequencing, array, and qPCR.

GLOBAL QUANTITATION
The Anti-5-hmC Polyclonal Antibody offers the greatest sensitivity for 5-hmC DNA available, and importantly demonstrates no crossreactivity with 5-mC modifications. It can be used successfully in immunodetection and immunopreciptation procedures requiring antibodies exhibiting high specificity. The Quest 5-hmC™ ELISA Kit features a streamlined workflow for rapid immunodetection and global quantitation of 5-hmC in DNA from any preparation.

DNA Degradase™ and DNA Degradase Plus™ enzymes are available for fast and accurate 5-mC and 5-hmC quantitation via chromatography and spectroscopy.

SITE-SPECIFIC DETECTION
5-hmC Glucosyltransferase can catalyze the addition of glucose to 5-hmC in DNA creating glucosyl-5-hmC. This can be used to differentiate between 5-hmC and 5-mC in DNA using glucosyl-sensitive endonucleases (MspI, MrcBC, etc.). The Quest 5-hmC™ Detection Kit consolidates these technologies for efficient site-specific detection of 5-hmC in DNA.

5-hmC Nucleotides & DNA Standards

Zymo Research offers a DNA Standard Set for 5-mC and 5-hmC quantitation applications (i.e. - mass spectrometry, HPLC, TLC, etc.). The DNA also serves as an excellent substrate for studies involving 5-hydroxymethylcytosine interacting proteins. Additionally dNTPs containing 5-hmC are available for generating amplicons for 5-hmC studies.

QuestTaq is a DNA polymerase that can be used for non-biased amplification of C, mC, and 5-hmC DNA in real-time PCR.

Genome-Wide 5-hmC Analysis Service

Genome-wide analysis of 5-hmC localization is readily attainable with our unique 5-hmC Enrichment/NextGen Sequencing service.

Please call or inquire at services@zymoresearch.com

 Cat. No.   Product   Size 
 5-hmC Enrichment/Detection  
D5420  Quest 5-hmC™ Enrichment Kit 25 preps
D5421  50 preps 
A4001-50  Anti-5-Hydroxymethylcytosine Polyclonal Antibody 50 µg
A4001-200  200 µg
D5425  Quest 5-hmC™ ELISA Kit 1 plate
D5426  2 plates
E2016  DNA Degradase™ 500 units
E2017  2,000 units
E2020  DNA Degradase Plus™ 250 units 
E2021  1,000 units 
E2026  5-hmC Glucosyltransferase 100 units 
E2027  200 units 
D5410  Quest 5-hmC™ Detection Kit 25 preps
D5411  50 preps 
 5-hmC Nucleotides & DNA Standards  
D5405  5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set 1 set 
D1040  5-Hydroxymethylcytosine dNTP Mix 10 mM 
D1045  5-Hydroxymethyl dCTP 100 mM 
D1030  5-Methylcytosine dNTP Mix 10 mM 
D1035  5-Methyl dCTP 10 mM 
E2050  QuestTaq™ PreMix 50 rxns 
E2051  200 rxns 
E2052  QuestTaq™ qPCR PreMix   50 rxns 
E2053  200 rxns