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ZymoBIOMICS™-96 MagBead DNA Kit

High throughput purification of high quality, inhibitor-free DNA from any sample including feces, soil, water, biofilms, swabs, saliva, and body fluids.
The ZymoBIOMICS™ innovative lysis system enables efficient and unbiased lysis of microbes including gram positive/negative bacteria, fungi, protozoans, algae, etc.
The automation friendly workflow enables nearly any sample to be processed in as little as 90 minutes for 96 preps

Product Size Catalog # Price Qty
ZymoBIOMICS™-96 MagBead DNA Kit (Includes ZR BashingBead™ Lysis Rack) 2 x96 Preps D4302
ZymoBIOMICS™-96 MagBead DNA Kit (Lysis Matrix Not Included) 2x96 preps D4306
ZymoBIOMICS™-96 MagBead DNA Kit (Includes ZR BashingBead™ Lysis Tubes) 2x96 preps D4308

About ZymoBIOMICS™-96 MagBead DNA Kit

The ZymoBIOMICS™-96 MagBead DNA Kit is designed for purifying DNA from a wide array of sample inputs (e.g. feces, soil, water, and biofilms) that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS™ innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. gram negative/positive bacteria, fungi, protozoans, and algae), making it ideal for microbiomic studies. Unbiased mechanical lysis of tough microbe s is achieved by bead beating with Zymo Research’s proprietary, ultra-high density BashingBeads™ and validated using the ZymoBIOMICS Microbial Community Standard as shown in Figure 3. Coupling this with Zymo Research’s Purification and PCR Inhibitor Removal Technologies result in superior yields of ultra-pure DNA that is free of PCR inhibitors (e.g. polyphenols, humic and fulvic acids), making it ideal for all downstream applications including PCR, arrays, 16s rRNA Gene Sequencing, and Shotgun Sequencing.

DNA Recovery Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum) ZymoBIOMICS™ DNase/RNase Free Water.
Equipment Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block
Sample Sources Bacterial, fungal, protozoan, algal, viral, mitochondrial, and host DNA is efficiently isolated from ≤ 100 mg of mammalian feces, ≤ 200 mg soil, and 5 – 20 mg (wet weight) of fungal/bacterial cells, biofilms, and water
DNA Purity High quality, inhibitor-free DNA is eluted with ZymoBIOMICS™ DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation Sequencing (NGS)
Bead Beating System The ZymoBIOMICS™ innovative lysis system enables complete homogenization/disruption of the microbial cell walls and accurate microbial DNA analysis, free of bias. To ensure unbiased lysis, calibration of each bead-beating device is recommended by using the ZymoBIOMICS™ Microbial Community Standard
DNA Integrity On average, post bead beating, genomic DNA is between 15-20 kb depending on the initial quality of the sample making it amenable to Next-Generation sequencing platforms requiring high molecular weight DNA. For optimal DNA integrity, collect samples in DNA/RNA Shield™
Bioburden A single preparation is guaranteed to contain less than 3 bacterial genomic copies per 1 µl of eluate, as determined by quantitative amplification of the 16S rRNA gene when eluted using 100 µl water

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