RNA Isolation Overview
The ZymoBIOMICS™ RNA Mini Kit is designed for purifying RNA, from a wide array of sample inputs (e.g. feces, soil, water, and biofilms), that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS™ innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative and positive bacteria, fungus, protozoans, spores and algae) making it ideal for microbial community profiling. Unbiased mechanical lysis of tough-to-lyse microbes is achieved by bead beating with the innovative ZymoBIOMICS™ ultra-high density BashingBeads™ and validated using the ZymoBIOMICS™ Microbial Community Standards. In addition, extracted RNA is inhibitor-free and ultra-pure thanks to Zymo’s proprietary OneStep™ PCR Inhibitor Removal and Zymo-Spin™ technologies. Purified RNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing.