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ZymoBIOMICS™ RNA Mini Kit

Rapid, robust, and simple purification of high quality, inhibitor-free total RNA (including small/micro RNAs) from any sample including feces, soil, water, biofilms, swabs, saliva, and body fluids, etc.
ZymoBIOMICS™ innovative lysis system enables efficient and unbiased lysis of microbes including gram positive/negative bacteria, fungus, protozoans, algae, viruses, etc.
DNA-free RNA is ready for use in any downstream application. DNase I included.

Product Size Catalog # Price Qty
ZymoBIOMICS™ RNA Mini Kit 50 Preps R2001
$425.00

About ZymoBIOMICS™ RNA Mini Kit

The ZymoBIOMICS RNA Mini Kit is designed for purifying RNA from a wide array of sample inputs (e.g. feces, soil, plant, water, and biofilms) that is ready for microbiome or metagenome analyses. The ZymoBIOMICS innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. gram negative/positive bacteria, fungus, protozoans, and algae). The provided DNA/RNA Shield preserves nucleic acids at ambient temperatures, providing an unbiased molecular snapshot of the sample. The procedure uses Zymo-Spin® column technology that results in high-quality total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors (e.g. polyphenols, humic acids, and fulvic acids) and is ready for RT-PCR, hybridization, sequencing, etc


Sample Sources Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells1, biofilms, and water.
RNA Purity A260/A280 >1.8, A260/A230 >1.8. DNase I provided for complete removal of DNA.
RNA Recovery The RNA binding capacity of the Zymo-Spin™ IIICG Column is ~100 µg.
RNA Storage RNA eluted with DNase/RNase-Free Water (provided) can be stored at ≤70ºC. The addition of RNase inhibitors in highly recommended for prolonged storage.
Equipment Needed Microcentrifuge, vortex, cell disrupter (recommended).
RNA Size RNAs ≥17 nucleotides.
Sample Preservation DNA/RNA Shield™ lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures.
Bead Beating System ZymoBIOMICS™ innovative lysis system ensures complete homogenization of the microbial cell walls and accurate microbial RNA analysis, free of bias.

      
       Human stool RNA isolated with the ZymoBIOMICS™ RNA Mini Kit is higher quality (right); compared to Supplier MB procedures (left). Quality assessed by Agilent 2200 TapeStation.
      
Human stool RNA was analyzed after RT-PCR and PCR amplification (~150 bp fragment shown) for both Zymo and Supplier MB procedures. Lack of a band in PCR using the ZymoBIOMICS™ RNA Mini Kit indicates DNA-free RNA. Quality assessed by Agilent 2200 TapeStation.        Total RNA isolated from human stool with or without inclusion of the Zymo-Spin™ IV-HRC Spin Filter during the ZymoBIOMICS™ RNA Mini Kit protocol. Earlier amplification cycles indicate complete removal of PCR inhibitors.
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