Zymo-Seq RiboFree Total RNA Library Kit
Comprehensive Gene Detection for Every Transcriptome & Beyond
The Zymo-Seq RiboFree Kit Detects an Exceptional Number of Genes. Total RNA extracted from various species were used as inputs for library preparation using a single standard RiboFree protocol. The libraries were paired-end sequenced. STAR-aligned reads were quantified at the gene level using featureCounts.
Maximize Your Sequencing Efficiency
>90% rRNA Depletion Across Organisms
The Zymo-Seq RiboFree Kit Produces Dense Coverage of Protein Coding and Other Transcripts. Classification of the STAR-aligned reads was based on Ensembl annotations and RepeatMasker rRNA tracks from UCSC genome browser when applicable.
Unparalleled Multi-Species Compatibility
RiboFree Universal Depletion Provides Cross-Species Compatibility. The Zymo-Seq RiboFree Total RNA Library Kit is unmatched in cross-species compatibility thanks to an innovative probe-free depletion strategy. Leveraging the robust depletion kinetics, scientists are exploring diverse applications of RiboFree using samples from vertebrates, plants and more.
Suitable for Degraded Samples*
The Zymo-Seq RiboFree Kit Effectively Depletes rRNA from Formalin-Fixed, Paraffin-Embedded (FFPE) Human Tissue RNA. Libraries were prepared with 250 ng, 100 ng, and 50 ng of total RNA extracted from FFPE tissues (RIN = 1.6, peak < 200 nt), respectively, with minimal modifications to the standard protocol as described in Appendix E of the instruction manual.
*Please contact tech@zymoresearch.com for additional recommendations.
The Fastest RNA-Seq Library Workflow
Sample to Sequencer in a Single Day
The Zymo-Seq RiboFree Kit has the Fastest Workflow from Total RNA to rRNA-depleted, NGS Libraries. The Zymo-Seq RiboFree Total RNA Library Kit offers a 20% - 40% reduction in workflow completion time compared to other available RNA library prep kits. Workflow time considers the hours required to complete both rRNA depletion and library preparation.
Featured Citations
1. S. Brochet et al., Niche partitioning facilitates coexistence of closely related honey bee gut bacteria. Elife 10, (2021).
2. J. Gray et al., Colonisation dynamics of Listeria monocytogenes strains isolated from food production environments. Sci Rep 11, 12195 (2021).
3. C. Yague-Sanz, M. Duval, M. Larochelle, F. Bachand, Co-transcriptional RNA cleavage by Drosha homolog Pac1 triggers transcription termination in fission yeast. Nucleic Acids Res 49, 8610-8624 (2021).
4. N. K. Sinha et al., EDF1 coordinates cellular responses to ribosome collisions. Elife 9, (2020).
5. K. V. Brussel et al., Identification of Novel Astroviruses in the Gastrointestinal Tract of Domestic Cats. Viruses 12, (2020).
6. D. Licastro et al., Isolation and Full-Length Genome Characterization of SARS-CoV-2 from COVID-19 Cases in Northern Italy. J Virol 94, (2020).
7. E. M. Wampande et al., Phylogenetic Characterization of Crimean-Congo Hemorrhagic Fever Virus Detected in African Blue Ticks Feeding on Cattle in a Ugandan Abattoir. Microorganisms 9, (2021).
8. D. O. Omelchenko et al., Assembly and Analysis of the Complete Mitochondrial Genome of Capsella bursa-pastoris. Plants (Basel) 9, (2020).
9. M. P. Swaffer et al., RNA polymerase II dynamics and mRNA stability feedback determine mRNA scaling with cell size. BioRxiv, (2021).