Pinpoint™ Slide RNA Isolation System I

 
• Allows for the isolation of total RNA from fresh tissue sections.
• Simple procedure combines Pinpoint™ tissue sampling technology with a one-step RNA extraction/purification method.
• Omits the use of organic denaturants.
 
Product Catalog No. Size Price Store
Pinpoint™ Slide RNA Isolation System I R1003 50 Preps. $
 
Components Available
 
• Format: Spin Column.
• Sample Sources: Cells from tissue sections on glass slides.
• RNA Recovery: Typically, up to 5 µg RNA is eluted into ≥ 8 µl RNase/DNase-free water allowing for a highly concentrated sample.
• RNA Storage: Recommended that 1 U/10 µl RNase inhibitor be added to the RNA prior to storage at -70°C.
• Processing Time: 1.5 hr
• Equipment Needed: Microcentrifuge
 

The Pinpoint™ Slide RNA Isolation System I is an innovative product for the isolation of RNA from any targeted area of fresh tissue sectioned onto a glass slide. The system combines powerful Pinpoint™ tissue sampling methodology, a unique single-step RNA extraction/binding buffer, and Fast-Spin column purification technology to yield high quality RNA. Unlike current UV-based methods, this product makes isolation of tissue RNA simple and quick. No expensive specialized equipment is needed. Eluted RNA is well suited for subsequent RNA analyses including RT-PCR.

 

RT-PCR of RNA recovered from human tissue using the Pinpoint™ RNA Isolation System. Amplicons (in duplicate) are from A) a human β-actin transcript; B) an arbitrary human transcript from Chromosome 3. M is 100 bp DNA Marker (Zymo Research).