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| • | Quick recovery of RNA from a wide range of cell types and tissue using Fast-Spin column technology. |
| • | Column design allows for elution of highly concentrated RNA. |
| • | Omits the use of organic denaturants and proteases. |
| Product | Catalog No. | Size | Price | Store |
|---|---|---|---|---|
| Quick-RNA™ MicroPrep | R1050 | 50 Preps. | $ | |
| R1051 | 200 Preps. | $ | ||
| Components Available | ||||
| • | Formats: Spin column. |
| • | Sample Sources: Cells from culture or tissue samples, buccal cells, buffy coat, plasma, serum, and other biological liquids. Not compatible with whole blood. (For purification of RNA from whole blood, see the ZR Whole-Blood RNA MiniPrep (R1020)) |
| • | Sample Size: 1 to 105 cells (≤100 μl plasma, serum). |
| • | RNA Recovery: Up to 5 μg RNA can be eluted into ≥ 6 μl RNase-free water allowing for a highly concentrated sample. |
| • | RNA Purity: High quality RNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations. |
| • | RNA Storage: 70ºC |
| • | Processing Time: 10 min |
| • | Equipment Needed: Microcentrifuge. |
The Quick-RNA™ MicroPrep is an innovative product designed for the easy, reliable, and rapid isolation of total RNA from cultured cells or solid tissue samples. The procedure combines a unique, single-step RNA extraction/binding buffer with Fast-Spin column technology to yield high quality RNA in minutes. The method is easy: simply add the provided ZR RNA Buffer to extract total RNA from the cells of interest and then purify the RNA using the provided spin column. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.
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Human RNA isolated from cell culture with the Quick-RNA™ MiniPrep Kit. M: 1 kb DNA marker (Zymo Research); 1, 2: duplicate RNA preparations.
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PCR amplification of a β-actin transcript (353 bp fragment shown) following RNA isolation from human epithelial cells (HCT 116) with the Quick-RNA™ MidiPrep: RT-PCR (lanes 1, 2), PCR negative control (RNA template; lanes 3, 4). M1 and M2 are 100 bp and 1 kb DNA Markers, respectively (Zymo Research).
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