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| • | For general cloning, blue-white selection, plasmid isolation. |
| • | Mix & Go! transformation procedure with transformation efficiencies of 108 - 109 transformants/µg of plasmid DNA. |
| • | Simple procedure: add DNA and then spread. DNA transformation in as little as 20 seconds! |
| Product | Catalog No. | Format | Price | Store |
|---|---|---|---|---|
| Z-Competent™ E. coli-JM109 | T3003 | 10 x 100 µl (10 tubes) |
$ | |
| T3005 | 96 x 50 µl (96-well plate) |
$ | ||
| • | Format: Either as a pack of ten 100 µl tube single use aliquots or as a 96-tube 50 µl format with removable 8-tube strips |
| • | Storage: -70°C |
| • | Genotype: F`[traD36 proA+B+ laclq ∆(lacZ)M15] ∆(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rk- mk+) relA1 recA1 |
| • | Additional Information: Partly restriction-deficient; good strain for cloning repetitive DNA (recA-). Suppresses many amber mutations when glutamine is available but not the S100 or S7 mutation of λ, e.g., λgt11. Can be used for M13 cloning/sequencing and blue/white screening. |
The Z-Competent™ E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Z-Competent™ E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Z-Competent™ cells are highly efficient (> 108 transformants / µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc. Premade Z-Competent™ cells are supplied as a pack of 10 convenient 100 µl/tube single use aliquots or in a 96-tube format with removable 8-tube strips for your high-throughput transformation needs.
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