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| • | Quick (20 minute) high-throughput recovery of ultra-pure DNA from agarose gels. |
| • | Column design permits DNA elution at high concentrations into minimal volumes (≥ 15 µl). |
| • | Eluted DNA is well suited for use in DNA ligation, sequencing, labeling, PCR, etc. |
| Product | Catalog No. | Size | Price | Store |
|---|---|---|---|---|
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ZR-96 Zymoclean™ Gel DNA Recovery Kit |
D4021 | 2x96 Preps. | $ | |
| D4022 | 4x96 Preps. | $ | ||
| Zymoclean™ Gel DNA Recovery Kit (uncapped columns) |
D4001 | 50 Preps. | $ | |
| D4002 | 200 Preps. | $ | ||
| Zymoclean™ Gel DNA Recovery Kit (capped columns) |
D4007 | 50 Preps. | $ | |
| D4008 | 200 Preps. | $ | ||
| Zymoclean™ Large Fragment DNA Recovery Kit | D4045 | 25 Preps. | $ | |
| D4046 | 100 Preps. | $ | ||
| Components Available | ||||
| • | Format: 96-well Plate (deep well) |
| • | Sample Sources: DNA excised from TAE/TBE-buffered agarose gels. |
| • | DNA Size Limits: 50 bp to 23 kb |
| • | DNA Recovery: Typically, up to 5 µg total DNA/well can be eluted with ≥ 15 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
| • | DNA Purity: High quality, purified DNA is eluted with water which is especially well suited for sequencing, ligation reactions, andrestriction endonuclease digestions. |
| • | Detergent Tolerance: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
| • | Processing Time: 20 min |
| • | Equipment Needed: Centrifuge w/ microplate carriers |
The ZR-96 Zymoclean™ Gel DNA Recovery Kit provides for the rapid purification of high quality DNA from TAE/TBE-buffered agarose gels. The product features Fast-Spin technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean™ Gel DNA Recovery kits are perfectly suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc.
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DNA fragments recovered from an agarose gel using the Zymoclean™ Gel DNA Recovery Kit. Lanes: M: DNA Ladder; 1-5: individual ladder DNA fragments.
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DNA sequencing chromoatogram of a PCR product recovered using the Zymoclean™ Gel DNA Recovery Kit. DNA was recovered from a 2% (w/v) agarose gel and used directly for sequencing.
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