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ZR Fungal/Bacterial RNA MiniPrep™
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Quick (15 minute) isolation of total RNA from tough-to-lyse bacteria, yeast, and fungi. |
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Omits the use of organic denaturants and proteases. |
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Fast-Spin column technology allows RNA to be eluted into minimal volumes (≥ 25 µl). |
| Product |
Catalog No. |
Size |
Price |
Store |
| ZR Fungal/Bacterial RNA MiniPrep™ |
R2014 |
50 Preps. |
$
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| Components Available
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Format: Spin Column |
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Sample Sources: Fresh or frozen pelleted cells (bacteria, yeast, fungi). |
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RNA Recovery: Up to 25 µg RNA can be eluted into ≥ 25 µl, allowing for a highly concentrated sample. |
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RNA Purity: High quality RNA (A260/A280>1.8, A260/A230>1.8) recovered. |
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RNA Storage: RNA is eluted with RNase-free water and can be stored at ≤ -70°C. The addition of RNase inhibitors is optional but highly recommended for prolonged storage. |
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Processing Time: 15 min |
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Equipment Needed: Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional). |
The ZR Fungal/Bacterial RNA MiniPrep™ provides for rapid isolation of total RNA from pelleted tough-to-lyse bacterial (e.g., Gram-positive), yeast, and/or fungal cells. The kit employs ultra-high density BashingBeads™ for sample homogenization and a robust buffer system for total RNA purification (small RNAs included). Using Fast-Spin column technology, the RNA is eluted into volumes as little as 25 µl and suitable for subsequent procedures including RT-PCR. The entire RNA isolation procedure typically takes less than 15 minutes.
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Total RNA was isolated from equal amounts of pelleted E. coli cells containing plasmid DNA (pGEM®) using the ZR Fungal/Bacterial RNA MicroPrep™ or kit from Supplier A. The samples were resolved in a 2% (w/v) agarose gel. RNA Millenium Markers (Ambion) and ZR 1 kb DNA Marker (Zymo Research) were used.
* = genomic (> 10 kb) and plasmid (> 3 kb) DNA contamination;
DNase I = samples treated with DNase I.
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