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| • | Hot start DNA Polymerase for robust product formation. |
| • | Reduces nonspecific PCR product formation from difficult templates (e.g., bisulfite-converted DNA). |
| • | Compatible with real-time, quantitative PCR and suitable for TA-cloning. |
| Product | Catalog No. | Size | Price | Store |
|---|---|---|---|---|
| ZymoTaq™ DNA Polymerase | E2001 | 50 Rxns. | $ | |
| Set: Hot start DNA Polymerase, 2X reaction buffer, dNTPs. | E2002 | 200 Rxns. | $ | |
| ZymoTaq™ PreMix | E2003 | 50 Rxns. | $ | |
| Single tube: 2X Hot start DNA Polymerase premix. | E2004 | 200 Rxns. | $ | |
| Components Available | ||||
| • | Format: Provided as a PreMix (E2003, E2004) or as a component of a set (E2001, E2002). |
| • | Source: Recombinant enzyme |
| • | Enzyme Concentration: Reaction conditions at 1X (50 µl total volume) contain 2 U of ZymoTaq™ DNA Polymerase. For the ZymoTaq™ PreMix (2X), the enzyme is at 4 U/50 µl. |
| • | Unit Definition: One unit (1U) is defined as the amount of enzyme required for the incorporation of 10 nM dNTPs into an acid-insoluble form in 30 minutes at 72°C. |
| • | Activity: 5' - 3' DNA polymerization |
| • | Optimum pH and Temperature: 72°C |
| • | Storage: Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at -80°C. |
ZymoTaq™ DNA Polymerase is a hot-start polymerase that is ideal for amplification of bisulfite-converted DNA. Because it is a heat-activated, thermostable DNA polymerase, ZymoTaq™ reduces primer dimer and nonspecific product formation, whereas conventional polymerases typically exhibit these problems with bisulfite-converted DNA templates. In addition to the amplification of bisulfite-treated DNA for methylation detection, ZymoTaq™ DNA polymerase can also be used for conventional PCR, and real time PCR. The enzyme also has 3′-terminal transferase activity, making it ideal for use in TA-cloning by the addition of “A” overhangs to amplified DNA.
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PCR products of immunoprecipitated, methylated DNA vary depending on the hot-start polymerase used. Methylated DNA was immunoprecipitated using the Methylated-DNA IP Kit. DNA (post-IP) was used in a PCR assay comparing Zymo Research’s hot-start ZymoTaq™ polymerase vs. that of three other suppliers (A, B, and C). Expected amplicon size is 350 bp. PCR products (in duplicate) were separated in a 2.0% (w/v) agarose TAE/EtBr gel. The use of ZymoTaq™ generated specific, robust products with minimal non-specific banding compared to others.
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ZymoTaq™ DNA Polymerase is available either as a single buffer premix or as a polymerase system with components provided separately.
