| »Make
your own Competent Cells |
| • |
Z-Competent
E. coli Transformation Buffer Set
(No heat-shock) High transformation efficiency: Easily reaches
over 108-109 transformants per ug of plasmid
DNA for many common lab strains. (Reagents provided make 60
ml of Z-Competent E. coli cells for transformation.
ZymoBroth™ is not included). |
• |
Z-Competent E.
coli Transformation Kit
High transformation efficiency: Easily reaches over 108-109
transformants per ug of plasmid DNA for many common lab strains.
(Reagents provided make 20 ml of Z-Competent E. coli
cells for transformation). |
• |
Rattler Plating Beads
Sterile and easy to use. Saves time, no flaming, and spread
cells more evenly. Great for libary plating, spread several
plates at once. |
• |
ZymoBroth
Specially formulated E. coli growth medium for making
highly growth medium for making highly competent E. coli
cells for transformation. Sterile. |
|
| »Chemically
Competent Strains |
| • |
Autolysis
Induce rapid and efficient lysis without sonication. |
• |
JM109
Total 4 mg at 10 mM in 0.1M sodium acetate pH 5.2, 240 ul. For
cell cycle and signal transduction studies. |
• |
DH5α
Z-Competent (No heat-shock). General cloning, blue-white selection,
plasmid isolation (slow growth, certain plasmids not stable),
transformation efficiency >108. |
• |
HB101
Z-Competent (No heat-shock). General cloning, plasmid isolation,
transformation efficiency >108. |
• |
C600
Z-Competent (No heat-shock). General cloning, plasmid isolation,
transformation efficiency >108. |
• |
TG1
Z-Competent (No heat-shock). General cloning, blue-white selection,
plasmid isolation, transformation efficiency >108. |
|
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