| »Transformation
& Media |
| • |
Frozen
EZ Yeast Transformation II
High transformation efficiency that yields approximately 105-106
transformants per ug plasmid DNA (circular). |
• |
YPD+
Increase overall transformation efficiency with YPD+ outgrowth. |
• |
Rattler™ Plating Beads
Sterile and easy to use. Saves time, no flaming, and spread
cells more evenly. Great for libary plating, spread several
plates at once. |
|
| »Genomic
DNA Purification |
| • |
YeaStar
Genomic DNA Kit
Highly efficient enzyme lysis and fast spin column technology
for isolation of pure yeast genomic DNA. No lysis beads or phenol
chloroform needed. |
|
| »Lytic
Enzyme |
| • |
Zymolyase
Our liquid yeast lytic enzyme is prepared from Arthrobacter
luteus, and is formulated for maximum activity. The primary
yeast lytic activity is B-1,3-glucan laminaripentaohydrolase.
It hydrolyzes glucose polymers at the B-1,3-glucan linkages
releasing laminaripentaose as the principal product. Optimal
temperature range for use of this enzyme is 30-37°C. Works
for spheroplasting. |
|
| »Mating
Pheromone |
| • |
Alpha-Factor
Mating Pheromone
Total 4 mg at 10 mM in 0.1M sodium acetate pH 5.2, 240 ul. For
cell cycle and signal transduction studies. |
|
| »Total
RNA Purification |
| • |
YeaStar
RNA Kit
RNA isolation from a broad spectrum of fungus species: Aspergillus
fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus,
Candida albicans, Pichia pastoris, Saccharomyces cerevisiae,
Schizosaccharomyces pombe, and any fungi whose cell walls are
susceptible to yeast lytic enzyme lysis. |
|
| »Yeast
Protein |
| • |
Yeast
Protein Kit
Simple and convenient method to make protein lysates for analysis,
such as Western Blot, as well as PCR amplification of plasmid
or genomic DNA. No lysis beads or phenol chloroform needed. |
|
| »Counterselection |
| • |
5FOA
Purity >98% by TLC. |
|
| »Plasmid
Rescue |
| • |
Zymoprep
I, Yeast Plasmid Miniprep
Efficient recovery of yeast plasmid: works for library plasmid
recovery from two-hybrid screens. |
• |
Zymoprep II, Yeast Plasmid Miniprep
More efficient recovery of yeast plasmid: spin column format
is ideal for low copy and hard to isolate plasmids. No precipitation
step. |
|
| »Other
Products |
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