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DNA
Clean & Concentrator™-100
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Simple, rapid recovery of ultra-pure DNA from PCR, endonuclease
digestions, “crude” DNA preps., etc. |
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Column
design allows DNA to be eluted at high concentrations
into minimal volumes of water or TE buffer. Unique column
construction allows sample washing to be performed using
a centrifuge, vacuum source, or syringe. |
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Column
design allows DNA to be eluted at high concentrations
into minimal volumes of water or TE buffer using a microcentrifuge. |
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Eluted
DNA is well suited for use in PCR, DNA sequencing, DNA
ligation, endonuclease digestion, RNA transcription, radiolabeling,
etc.
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SPECIFICATIONS
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Format: Spin Column. |
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DNA
Purity: Highly purified DNA is eluted with water and
is especially well suited for sequencing, ligation reactions,
and restriction endonuclease digestions. |
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DNA
Size Limits: From 50 bp to 23 kb. |
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DNA
Recovery: Typically, ≤ 100 µg total DNA
can be eluted with ≥ 100 µl water or low salt elution
buffer. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA
11 kb to 23 kb the recovery is 50-70%. |
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Sample
Sources: DNA from PCR, restriction endonuclease digestions,
“crude” DNA preparations, etc. |
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Product
Detergent Tolerance: ≤ 5% Triton X-100, ≤
5% Tween-20, ≤ 5% Sarkosyl, ≤ 0.1% SDS. |
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Equipment:
Microcentrifuge and centrifuge, vacuum source, or syringe. |
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| DESCRIPTION
The DNA Clean & Concentrator™-100
was designed for the rapid purification and concentration
of up to 100 µg of high quality DNA from PCR, large
format restriction endonuclease digestions, or "crude"
DNA preparations. The DCC™-100 features a single-buffer
system that allows for efficient DNA adsorption onto the
matrix of the supplied Zymo-Spin™ column. The DNA
is washed twice using either a centrifuge, vacuum source,
or syringe then eluted with a small volume (≥ 100
µl) of water using a microcentrifuge. The procedure
is easy: simply add the specially formulated DNA Binding
Buffer to your sample then transfer the mixture to the supplied
to your sample then transfer the mixture to the supplied
Zymo-Spin™ column. There is no need for organic denaturants
or chloroform. Instead, the product features Fast-Spin column
technology to yield high quality, purified DNA in just minutes.
DNA purified using the DCC™-100 is suitable for DNA
sequencing, DNA transfection, RNA transcription, microarray
analysis, PCR, nucleotide blotting, restriction endonuclease
digestion, etc.
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| DNA
sequencing chromatogram of DNA isolated using the DNA
Clean & Concentrator™. |
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ORDERING INFORMATION:
| Products |
Description |
Protocol |
Cat
No. |
Size |
Price |
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DNA
Clean &
Concentrator™-100
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For
25 DNA preps. |
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D4029 |
1
kit |
$82.00 |
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| For
50 DNA preps. |
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D4030 |
1
kit |
$142.00 |
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| Components
Available Separately |
| DNA
Binding Buffer |
D4004-1-L |
100
ml |
$49.00 |
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| DNA
Wash Buffer |
D4003-2-24 |
24
ml |
$28.00 |
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| D4003-2-48 |
48
ml |
$52.00 |
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| Zymo-Spin
V Columns |
C1012-25 |
25
columns |
$30.00 |
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RELATED PRODUCTS:
| Products |
Description |
Cat
No. |
Size |
Price |
|
DNA
Clean &
Concentrator™-5
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For
50 DNA preps. (Supplied with uncapped columns) |
D4003 |
1
kit |
$62.00 |
|
| For
200 DNA preps. (Supplied with uncapped columns) |
D4004 |
1
kit |
$220.00 |
|
For
50 DNA preps. (Supplied with capped columns)
|
D4013 |
1
kit |
$62.00 |
|
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For 200 DNA preps. (Supplied with capped columns) |
D4014 |
1
kit |
$220.00 |
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