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DNA
Clean & Concentrator™-500
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Simple, rapid recovery of ultra-pure DNA from large-scale
sample sources. |
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Column
design allows DNA to be eluted at high concentrations
into minimal volumes of water or TE buffer. Unique column
construction allows sample washing to be performed using
a centrifuge or vacuum source. |
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Eluted
DNA is well suited for use in PCR, DNA sequencing, DNA
ligation, endonuclease digestion, RNA transcription, radiolabeling,
etc.
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SPECIFICATIONS
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Format: Spin Column. |
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DNA
Purity: Highly purified DNA is eluted with water and
is especially well suited for sequencing, ligation reactions,
and restriction endonuclease digestions. |
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DNA
Size Limits: From 50 bp to 23 kb. |
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DNA
Recovery: Typically, ≤ 500 µg total DNA
can be eluted with ≥ 1 ml water or low salt elution buffer.
For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb
to 23 kb the recovery is 50-70%. |
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Sample
Sources: DNA from large-scale sample sources including
restriction endonuclease digestions and "crude" DNA
preparations from cell-free lysates. |
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Product
Detergent Tolerance: ≤ 5% Triton X-100, ≤
5% Tween-20, ≤ 5% Sarkosyl, ≤ 0.1% SDS. |
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Equipment:
Microcentrifuge and centrifuge, vacuum source, or syringe. |
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| DESCRIPTION
The DNA Clean & Concentrator™-500
was designed for the rapid, large format purification and
concentration of up to 500 µg of high quality DNA
from samples including large-scale restriction endonuclease
digestions and "crude" DNA preparations. The DCC™-500
features a single buffer system that allows for efficient
DNA adsorption onto the matrix of the supplied Zymo-Spin™
column. The DNA is washed twice using a centrifuge or vacuum
source then eluted with a small volume (≥ 1 ml) of
water using a centrifuge. The procedure is easy: simply
add the specially formulated DNA Binding Buffer to your
sample then transfer the mixture to the supplied to your
sample then transfer the mixture to the supplied Zymo-Spin™
column. There is no need for organic denaturants or chloroform.
Instead, the product features unique column technology to
yield high quality, purified DNA in just minutes. DNA purified
using the DCC™-500 is suitable for DNA sequencing,
DNA transfection, RNA transcription, microarray analysis,
PCR, nucleotide blotting, restriction endonuclease digestion,
etc.
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ORDERING INFORMATION:
| Products |
Description |
Protocol |
Cat
No. |
Size |
Price |
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DNA
Clean &
Concentrator™-500
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For
10 DNA preps. |
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D4031 |
1
kit |
$62.00 |
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| For
20 DNA preps. |
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D4032 |
1
kit |
$110.00 |
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| Components
Available Separately |
| DNA
Binding Buffer |
D4004-1-L |
100
ml |
$49.00 |
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| DNA
Wash Buffer
(concentrate) |
D4003-2-24 |
24
ml |
$28.00 |
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| D4003-2-48 |
48
ml |
$52.00 |
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| Zymo-Spin
VI Columns |
C1013-10 |
10
columns |
$30.00 |
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RELATED PRODUCTS:
| Products |
Description |
Cat
No. |
Size |
Price |
|
DNA
Clean &
Concentrator™-5
|
For
50 DNA preps. (Supplied with uncapped columns) |
D4003 |
1
kit |
$62.00 |
|
| For
200 DNA preps. (Supplied with uncapped columns) |
D4004 |
1
kit |
$220.00 |
|
For
50 DNA preps. (Supplied with capped columns)
|
D4013 |
1
kit |
$62.00 |
|
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For 200 DNA preps. (Supplied with capped columns) |
D4014 |
1
kit |
$220.00 |
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| Ultra-pure
DNA from the DNA Clean & Concentrator™
can be easily ligated for efficient cloning. Lanes: A: 3.5 kb blunt-ended
vector; B: 7.4 kb blunt-ended DNA insert; C: Blunt-end ligation
of A+B mixture. |
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