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EZ-96
DNA Methylation-Direct™
Kit (Shallow-Well Format)
• |
High throughput (96-well), complete bisulfite conversion
of DNA directly* from blood, tissue, or cells. |
| • |
Compatible with small sample inputs – as few as
10 cells or 50 pg DNA. |
| • |
Well-suited
for FFPE and LCM-derived samples.
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SPECIFICATIONS
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• |
Cells: Compatible with cells from solid tissue,
tissue culture, whole blood, buffy coat, biopsies, LCM (Laser-Capture
Micro-Dissection) and FFPE samples, etc. The number of cells
per standard treatment can range from 10 - 105 cells.
For optimal results, the cell number should be from 1 x 103
- 8 x 104 cells. |
|
• |
Purified DNA: Samples containing
50 pg - 2 µg of DNA. For optimal results, the amount of
input DNA should be from 200 to 500 ng. |
| • |
Conversion
Efficiency: > 99.5% of non-methylated C
residues are converted to U; > 99.5% protection of methylated
cytosines. |
| • |
DNA
Recovery: > 80% |
| • |
Sensitivity
of Detection (Lower
Limit): 10 cells for successful PCR amplification. |
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DESCRIPTION
The EZ
DNA Methylation-Direct™ Kit is a further
refinement of our popular EZ
DNA Methylation™ and EZ
DNA Methylation-Gold™ Kits. The EZ
DNA Methylation-Direct™ Kit features
simple and reliable DNA bisulfite conversion directly from
blood, tissue, and cells without the prerequisite for DNA
purification. The increased sensitivity of this kit makes
it possible to amplify bisulfite converted DNA from as few
as 10 cells or 50 pg DNA. Like the EZ
DNA Methylation-Gold™ Kit, DNA denaturation
and bisulfite conversion processes are combined into a single
step (see below). All kits streamline the three step process
of bisulfite conversion of non-methylated cytosine in DNA
into uracil. In addition the methylation kits share innovative
in-column desulphonation technology that eliminates otherwise
cumbersome DNA precipitation steps while ensuring researchers
consistent results every time. The kits have been designed
to minimize template degradation, loss of DNA during treatment
and clean-up, and to provide complete conversion of unmethylated
cytosines. Recovered DNA is ideal for PCR amplification
for downstream analyses including restriction endonuclease
digestion, sequencing, microarrays, etc.
An outline comparing the EZ
DNA Methylation-Direct™ Kit procedure
to Zymo Research’s other methylation
kits is shown below.
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ORDERING
INFORMATION:
| Products |
Protocol |
Cat
No. |
Size |
Price |
|
EZ-96
DNA Methylation-Direct Kit™
Shallow-Well (2 x 96 Rxns.)
|
|
D5022 |
1
set |
$362.00 |
|
EZ-96
DNA Methylation-Direct™ Kit
Deep-Well (2 x 96 Rxns.) |
|
D5023 |
1
kit |
$362.00 |
|
|
Components
Available Separately |
| CT
Conversion Reagent |
D5001-1 |
10
Conv. |
$6.10 |
|
| D5003-1 |
96
Conv. |
$55.00 |
|
| M-Dilution
Buffer-Gold |
D5005-2 |
1.5
ml |
$3.00 |
|
| D5006-2 |
7
ml |
$6.50 |
|
| M-Binding
Buffer-Gold |
D5005-3 |
30
ml |
$17.00 |
|
| D5006-3 |
125
ml |
$50.00 |
|
| M-Wash
Buffer |
D5001-4 |
6
ml |
$7.00 |
|
| D5002-4 |
24
ml |
$21.00 |
|
| D5007-4 |
36
ml |
$32.00 |
|
| M-Desulphonation
Buffer |
D5001-5 |
10
ml |
$12.00 |
|
| D5002-5 |
40
ml |
$36.00 |
|
| M-Elution
Buffer |
D5001-6 |
1
ml |
$1.50 |
|
| D5002-6 |
4
ml |
$4.50 |
|
| M-Solubilization
Buffer |
D5020-7 |
4.5
ml |
$16.00 |
|
| D5021-7 |
18
ml |
$24.00 |
|
| M-Reaction
Buffer |
D5020-8 |
1
ml |
$12.00 |
|
| D5021-8 |
4 ml |
$18.00 |
|
| M-Digestion
Buffer |
D5020-9 |
4
ml |
$5.00 |
|
| D5021-9 |
15
ml |
$12.00 |
|
| Proteinase
K and Storage Buffer |
D3001-2-5 |
5
mg set |
$18.00 |
|
| D3001-2-20 |
20
mg set |
$38.00 |
|
| Zymo-Spin
IC Columns |
C1004-50
|
50
pk |
$46.00 |
|
| C1004-250
|
250
pk |
$197.00 |
|
| Collection
Tubes |
C1001-50 |
50
pk |
$5.00 |
|
| C1001-500 |
500
pk |
$30.00 |
|
| C1001-1000 |
1,000
pk |
$50.00 |
|
| Zymo
Spin I-96 Plates |
C2004 |
2
pk |
$124.00 |
|
| ZR-96
Silicon A Plates |
C2001 |
2
pk |
$112.00 |
|
| ZR-96
Reaction Plates |
C2005 |
2
pk |
$5.00 |
|
| ZR-96
Collection Plates |
C2002 |
2
pk |
$18.00 |
|
| ZR-96
Elution Plates |
C2003 |
2
pk |
$15.00 |
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RELATED PRODUCTS:
| Products |
Cat
No. |
Size |
Price |
|
EZ
DNA Methylation™ Kit
For 50 DNA reactions.
(Supplied with capped columns)
|
D5001 |
1
kit |
$112.00 |
|
EZ
DNA Methylation™ Kit
For 200 DNA reactions.
(Supplied with capped columns)
|
D5002 |
1
kit |
$390.00 |
|
EZ-96 DNA Methylation™ Kit
For 2x96 DNA conversion reactions.
(Shallow-well Silicon-A™ Plate)
|
D5003 |
1
kit |
$310.00 |
|
EZ-96
DNA Methylation™ Kit
For 2x96 DNA conversion reactions.
(Deep-well Zymo-Spin™ I-96 Plate) |
D5004 |
1
kit |
$310.00 |
|
EZ
DNA Methylation-Gold™ Kit
For 50 DNA preps.
(Supplied with capped columns)
|
D5005 |
1
kit |
$121.00 |
|
EZ
DNA Methylation-Gold™ Kit
For 200 DNA preps.
(Supplied with capped columns)
|
D5006 |
1
kit |
$410.00 |
|
EZ-96
DNA Methylation-Gold™
Kit
For 2x96 DNA conversion reactions.
(Shallow-well Silicon-A Plate) |
D5007 |
1
kit |
$320.00 |
|
EZ-96
DNA Methylation-Gold™
Kit
For 2x96 DNA conversion reactions.
(Deep-well Zymo-Spin™ I-96 Plate) |
D5008 |
1
kit |
$320.00 |
|
Universal
Methylated DNA Standard
Methylated DNA standard for use with
Zymo Research’s EZ DNA Methylation™,
EZ DNA Methylation-Gold™, and EZ DNA
Methylation-Direct™ Kits.
|
D5010 |
1
set |
$122.00 |
|
Universal
Methylated Human DNA Standard
Methylated Human DNA standard for use
with Zymo Research’s EZ DNA Methylation™,
EZ DNA Methylation-Gold™, and EZ DNA
Methylation-Direct™ Kits.. |
D5011 |
1
set |
$182.00 |
|
Universal
Methylated Mouse DNA Standard
Methylated Mouse DNA standard for use
with Zymo Research’s EZ DNA Methylation™,
EZ DNA Methylation-Gold™, and EZ DNA
Methylation-Direct™ Kits. |
D5012 |
1
set |
$182.00 |
 |
EZ
DNA Methylation-Direct™ Kit
For 50 DNA preps. |
D5020 |
1
kit |
$161.00 |
|
EZ
DNA Methylation-Direct Kit
For 200 DNA preps. |
D5021 |
1
kit |
$452.00 |
|
EZ
DNA Methylation-Direct™ Kit*
(Free Kit Offer - 50 Rxns.) |
D5020S |
1
kit |
FREE |
|
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*Free kit offer
valid online only and while supplies last. Limit 1 per U.S.
customer only. |
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Download
Data Sheet
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