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EZ-96
DNA Methylation Kit™
(Shallow Well Format)
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High throughput (96-well), proven procedure for bisulfite
conversion of DNA. |
| • |
96-well desulphonation and recovery of bisulfite-treated
DNA. |
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Recovered
DNA is ideal for downstream analyses including PCR, endonuclease
digestion, sequencing, microarrays, etc.
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SPECIFICATIONS
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Format: Chemical denaturation of DNA followed
by sodium bisulfite modification, Spin Column. |
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Recovery
Volume: Purified, bisulfite treated DNA is
recovered in ≥ 10 µl provided elution buffer. |
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Input
DNA: 500 pg - 2 µg DNA per treatment
with 200 - 500 ng being optimal. |
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Sample
Sources: Plasmid, genomic, and endonuclease
digested DNAs. |
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Equipment:
Microcentrifuge (EZ DNA Methylation Kit™) centrifuge w/
microplate carriers (EZ-96 DNA Methylation Kit™). |
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DESCRIPTION
The EZ-96 DNA Methylation™ Kit features
a high-throughput (96-well), simplified procedure that streamlines
bisulfite conversion of DNA. The kit is based on the three-step
reaction that takes place between cytosine and sodium bisulfite
where cytosine is converted into uracil. The product’s
innovative desulphonation technology eliminates otherwise
cumbersome precipitations. The kit is designed to reduce
template degradation, minimize DNA loss during treatment
and clean-up, while ensuring complete conversion of the
DNA. Purified, converted DNA is ideal for PCR amplification
for downstream analyses including endonuclease digestion,
sequencing, microarrays, etc. An outline comparing the EZ
DNA Methylation™ Kit procedure to Zymo Research’s
other methylation kits is shown to the right. |
ORDERING INFORMATION:
| Products |
Protocol |
Cat
No. |
Size |
Price |
|
EZ-96
DNA Methylation™ Kit
For 2x96 DNA conversion reactions.
(Shallow-well Silicon-A Filter Plate™)
|
|
D5003 |
1
kit |
$310.00 |
|
EZ-96
DNA Methylation™ Kit
For 2x96 DNA conversion reactions.
(Deep-well Zymo-Spin I-96 Filter Plate™)
|
|
D5004 |
1
kit |
$310.00 |
|
EZ
DNA Methylation Kit™
For 50 DNA reactions.
(Supplied with capped columns) |
|
D5001 |
1
kit |
$112.00 |
|
EZ
DNA Methylation Kit™
For 200 DNA reactions.
(Supplied with capped columns) |
|
D5002 |
1
kit |
$390.00 |
|
Universal
Methylated DNA Standard
Methylated DNA standard for use with Zymo Research’s
EZ DNA Methylation™, EZ DNA Methylation-Gold™,
and EZ DNA Methylation-Direct™ Kits.
|
|
D5010 |
1
set |
$122.00 |
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Universal
Methylated Human DNA Standard
Methylated Human DNA standard for use with Zymo Research’s
EZ DNA Methylation™, EZ DNA Methylation-Gold™,
and EZ DNA Methylation-Direct™ Kits.. |
|
D5011 |
1
set |
$182.00 |
|
Universal
Methylated Mouse DNA Standard
Methylated Mouse DNA standard for use with Zymo
Research’s EZ DNA Methylation™, EZ DNA Methylation-Gold™,
and EZ DNA Methylation-Direct™ Kits. |
|
D5012 |
1
set |
$182.00 |
 |
|
| Components
Available Separately |
| CT
Conversion Reagent |
D5001-1 |
10
Conv. |
$6.10 |
|
| D5003-1 |
96
Conv. |
$55.00 |
|
| M-Dilution
Buffer |
D5001-2 |
1.3
ml |
$2.00 |
|
| D5002-2 |
5.2
ml |
$6.00 |
|
| M-Binding
Buffer |
D5001-3 |
20
ml |
$11.00 |
|
| D5002-3 |
80
ml |
$33.00 |
|
| M-Wash
Buffer |
D5001-4 |
6
ml |
$7.00 |
|
| D5002-4 |
24
ml |
$21.00 |
|
| D5007-4 |
36
ml |
$32.00 |
|
| M-Desulphonation
Buffer |
D5001-5 |
10
ml |
$12.00 |
|
| D5002-5 |
40
ml |
$36.00 |
|
| M-Elution
Buffer |
D5001-6 |
1
ml |
$1.50 |
|
| D5002-6 |
4
ml |
$4.50 |
|
| Zymo-Spin
IC Columns
(capped) |
C1004-50
|
50
columns |
$46.00 |
|
| C1004-250
|
250
columns |
$197.00 |
|
| Collection
Tubes |
C1001-50 |
50
tubes |
$5.00 |
|
| C1001-500 |
500
tubes |
$30.00 |
|
| C1001-1000 |
1,000
tubes |
$50.00 |
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| Zymo-Spin
I-96 Filtration Plates |
C2004 |
2
plates |
$124.00 |
|
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Silicon-A Plates |
C2001 |
2
plates |
$112.00 |
|
| Conversion
Plates w/Piercable
Cover Film |
C2005 |
2
plates |
$5.00 |
|
| Collection
Plates |
C2002 |
2
plates |
$18.00 |
|
| Elution
Plates |
C2003 |
2
plates |
$15.00 |
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RELATED PRODUCTS:
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Download
Data Sheet
A
459 bp PCR product amplified from DNA converted with the EZ
DNA Methylation Kit™ (lane 1) and from DNA not
treated with the kit (lane 2) was digested with EcoR I. The cytosine
at the EcoR I site was converted to uracil during treatment, not
resulting in cleavage of the DNA by the endonuclease. |
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