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EZ
DNA Methylation-Gold Kit™
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Complete bisulfite conversion of GC-rich DNA in less than
3 hours. |
| • |
A coupled DNA denaturation/bisulfite treatment step streamlines
the conversion of unmethylated cytosines into uracil. |
| • |
DNA precipitations are omitted. Instead, DNA is cleaned
and desulphonated in a single step using state-of-the-art
spin columns. |
| • |
Eluted, ultra-pure DNA is ideal for use in subsequent
molecular-based analyses.
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SPECIFICATIONS
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Format: Rapid heat denaturation of DNA followed
by sodium bisulfite modification, Spin Column. |
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Recovery Volume: Purified,
bisulfite treated DNA is recovered in ≥ 10 µl provided
elution buffer. |
| • |
Input
DNA: 500 pg - 2 µg DNA per treatment
with 200 - 500 ng being optimal. |
| • |
Sample
Sources: Plasmid, genomic, and endonuclease
digested DNAs. |
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Equipment:
Microcentrifuge and thermalcycler, centrifuge w/ microplate
carriers (EZ-96 DNA Methylation-Gold Kit™) |
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| DESCRIPTION
The EZ DNA Methylation-Gold Kit™
is a refinement of our popular EZ DNA Methylation Kit™.
The EZ DNA Methylation-Gold Kit™ integrates the DNA
denaturation and bisulfite conversion processes into one
step. This is accomplished using temperature denaturation
to replace chemical denaturation with sodium hydroxide in
the previous protocol. Also, the kit has been streamlined
for high yield recovery of DNA following bisulfite treatment.
Both kits are based on a three step reaction process between
cytosine and sodium bisulfite resulting in cytosine being
converted into uracil. The EZ DNA Methylation-Gold™
and EZ DNA Methylation Kits™ share innovative in-column
desulphonation technology that eliminates cumbersome DNA
precipitation steps while providing researchers consistent
results every time. The kits have been designed to minimize
template degradation, loss of DNA during treatment and clean-up,
and to provide complete conversion of unmethylated cytosines.
Recovered DNA is ideal for PCR amplification for downstream
analyses including restriction endonuclease digestion, sequencing,
microarrays, etc. The entire procedure can be completed
in less than 3 hours.
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| DNA
sequencing results after bisulfite treatment. DNA with
methylated CmpG (at nucleotide position #5) was processed
using the EZ DNA Methylation-Gold Kit™.
The recovered DNA was amplified by PCR and then sequenced
directly. The methylated cytosine at position #5 remained
intact while the unmethylated cytosines (i.e., positions
#7, 9, 11, 14 and 15) were completely converted into
uracil following bisulfite treatment and were detected
as thymine following PCR. |
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ORDERING INFORMATION:
| Products |
Protocol |
Cat
No. |
Size |
Price |
|
EZ
DNA Methylation-Gold Kit™
For 50 DNA preps.
(Supplied with capped columns)
|
|
D5005 |
1
kit |
$121.00 |
|
EZ
DNA Methylation-Gold Kit™
For 200 DNA preps.
(Supplied with capped columns)
|
|
D5006 |
1
kit |
$410.00 |
|
EZ-96
DNA Methylation-Gold Kit™
For 2x96 DNA conversion reactions.
(Shallow-well Silicon-A Filter Plate™) |
|
D5007 |
1
kit |
$320.00 |
|
EZ-96
DNA Methylation-Gold Kit™
For 2x96 DNA conversion reactions.
(Deep-well Zymo-Spin I-96 Filter Plate™)
|
|
D5008 |
1
set |
$320.00 |
|
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| Components
Available Separately |
| CT
Conversion Reagent |
D5001-1 |
10
Conv. |
$6.10 |
|
| D5003-1 |
96
Conv. |
$55.00 |
|
| M-Dilution
Buffer |
D5001-2 |
1.3
ml |
$2.00 |
|
| D5002-2 |
5.2
ml |
$6.00 |
|
| M-Binding
Buffer |
D5001-3 |
20
ml |
$11.00 |
|
| D5002-3 |
80
ml |
$33.00 |
|
| M-Wash
Buffer |
D5001-4 |
6
ml |
$7.00 |
|
| D5002-4 |
24
ml |
$21.00 |
|
| D5007-4 |
36
ml |
$32.00 |
|
| M-Desulphonation
Buffer |
D5001-5 |
10
ml |
$12.00 |
|
| D5002-5 |
40
ml |
$36.00 |
|
| M-Elution
Buffer |
D5001-6 |
1
ml |
$1.50 |
|
| D5002-6 |
4
ml |
$4.50 |
|
| M-Dissolving
Buffer-Gold |
D5005-6 |
500
µl |
$5.00 |
|
| D5006-6 |
1.2
ml |
$12.00 |
|
| Zymo-Spin
IC Columns
(capped) |
C1004-50
|
50
columns |
$46.00 |
|
| C1004-250
|
250
columns |
$197.00 |
|
| Collection
Tubes |
C1001-50 |
50
tubes |
$5.00 |
|
| C1001-500 |
500
tubes |
$30.00 |
|
| C1001-1000 |
1,000
tubes |
$50.00 |
|
| Zymo-Spin
I-96 Filtration Plates |
C2004 |
2
plates |
$124.00 |
|
|
Silicon-A Plates |
C2001 |
2
plates |
$112.00 |
|
| Conversion
Plates w/Piercable
Cover Film |
C2005 |
2
plates |
$5.00 |
|
| Collection
Plates |
C2002 |
2
plates |
$18.00 |
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| Elution
Plates |
C2003 |
2
plates |
$15.00 |
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RELATED PRODUCTS:
| Products |
Cat
No. |
Size |
Price |
|
EZ
DNA Methylation™ Kit
For 50 DNA reactions.
(Supplied with capped columns)
|
D5001 |
1
kit |
$112.00 |
|
EZ
DNA Methylation™ Kit
For 200 DNA reactions.
(Supplied with capped columns)
|
D5002 |
1
kit |
$390.00 |
|
EZ-96 DNA Methylation™ Kit
For 2x96 DNA conversion reactions.
(Shallow-well Silicon-A™ Plate)
|
D5003 |
1
kit |
$310.00 |
|
EZ-96
DNA Methylation™ Kit
For 2x96 DNA conversion reactions.
(Deep-well Zymo-Spin™ I-96 Plate) |
D5004 |
1
kit |
$310.00 |
|
Universal
Methylated DNA Standard
Methylated DNA standard for use with
Zymo Research’s EZ DNA Methylation™,
EZ DNA Methylation-Gold™, and EZ DNA
Methylation-Direct™ Kits.
|
D5010 |
1
set |
$122.00 |
|
Universal
Methylated Human DNA Standard
Methylated Human DNA standard for use
with Zymo Research’s EZ DNA Methylation™,
EZ DNA Methylation-Gold™, and EZ DNA
Methylation-Direct™ Kits.. |
D5011 |
1
set |
$182.00 |
|
Universal
Methylated Mouse DNA Standard
Methylated Mouse DNA standard for use
with Zymo Research’s EZ DNA Methylation™,
EZ DNA Methylation-Gold™, and EZ DNA
Methylation-Direct™ Kits. |
D5012 |
1
set |
$182.00 |
 |
EZ
DNA Methylation-Direct™ Kit
For 50 DNA preps. |
D5020 |
1
kit |
$161.00 |
|
EZ
DNA Methylation-Direct Kit
For 200 DNA preps. |
D5021 |
1
kit |
$452.00 |
|
EZ-96
DNA Methylation-Direct™
Kit
For 2x96 DNA conversion reactions.
(Shallow-well Silicon-A™ Plate) |
D5022 |
1
kit |
$362.00 |
|
EZ-96
DNA Methylation-Direct™
Kit
For 2x96 DNA conversion reactions.
(Deep-well Zymo-Spin™ I-96 Plate) |
D5023 |
1
kit |
$362.00 |
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Download
Data Sheet |
