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Universal
Methylated DNA Standard & Control Primers
Description
The Universal Methylated DNA Standard includes
enzymatically methylated DNA together with a specially-designed
primer set to be used in conjunction with Zymo Research’s
EZ DNA Methylation™, EZ DNA Methylation-Gold™,
and EZ DNA Methylation-Direct™ Kits to assess
the efficiency of bisulfite-mediated conversion of DNA. Central
to this, is the pUC19 DNA that was isolated from a methylation-negative
strain of bacteria (dam-, dcm-) prior to its enzymatic
modification with Sss I methylase (EC 2.1.1.73). The DNA is methylated
at cytosine positions comprising CG dinucleotides. The primer set
herein has been designed to amplify a fragment of the supplied pUC19
DNA following bisulfite treatment. The methylated cytosines comprising
CG dinucleotides remain unconverted following bisulfite treatment,
whereas nonmethylated cytosines
are converted into uracil and detected
as thymine following PCR.
Methylation
Site:
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Universal
Methylated DNA Standard, 20 µl. This standard
contains pUC19 DNA, which contains methylated cytosines (C5)
at all CG dinucleotide positions.
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SPECIFICATIONS
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Format: Used as a control with Zymo Research’s
EZ DNA Methylation™, EZ DNA Methylation-Gold™, and
EZ DNA Methylation-Direct™ Kits. |
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Control
DNA: 5 pg/µl of universal methylated pUC19 DNA
and 250 ng/µl of salmon sperm DNA as carrier in TE buffer
(10 mM Tris-HCl, 1 mM EDTA, pH8.0). |
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Primers:
Two control primers are supplied at a concentration of 20 µM
in 20 µl TE. |
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1
Set can be used for up to 20 separate bisulfite treatments
with either the EZ DNA Methylation Kit™ or the EZ DNA
Methylation-Gold Kit™. |
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ORDERING
INFORMATION:
| Products |
Protocol |
Cat
No. |
Size |
Price |
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Universal
Methylated DNA Standard
Methylated DNA standard for use with Zymo Research’s
EZ DNA Methylation™, EZ DNA Methylation-Gold™,
and EZ DNA Methylation-Direct™ Kits. |
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D5010 |
1
set |
$122.00 |
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RELATED
PRODUCTS:
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Download
Spec Sheet
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