The Universal Methylated Human DNA Standard includes
enzymatically methylated DNA together with a specially-designed
primer set to be used in conjunction with Zymo Research’s
EZ DNA Methylation™, EZ DNA Methylation-Gold™,
and EZ DNA Methylation-Direct™ Kits to assess
the efficiency of bisulfite-mediated conversion of DNA. The supplied
human DNA is methylated at all cytosine positions comprising CG
dinucleotides by Sss I methylase (EC 2.1.1.73). The primer set herein
is designed to amplify a fragment of the human MLH1 mismatch repair
gene following bisulfite treatment. The methylated cytosines comprising
CG dinucleotides remain unconverted following bisulfite treatment,
whereas nonmethylated cytosines are converted into uracil and detected
as thymine following PCR.
Methylation
Site:
Universal
Methylated Human DNA Standard, 5 µg/20 µl.
Source: DNA derived from male human liver.
DNA has methylated cytosines (C5) at all CG dinucleotide positions.
SPECIFICATIONS
•
Format: Used as a control with Zymo Research’s
EZ DNA Methylation™, EZ DNA Methylation-Gold™, and
EZ DNA Methylation-Direct™ Kits.
•
Control
DNA: 250 ng/µl in TE buffer (10 mM Tris-HCl,
1 mM EDTA, pH 8.0)
•
Primers:
Two control primers are supplied at a concentration of 20 µM
in 20 µl TE.
•
1
Set can
be used for up to 20 separate bisulfite treatments with either
the EZ DNA Methylation Kit™ or the EZ DNA Methylation-Gold
Kit™.
ORDERING
INFORMATION:
Products
Protocol
Cat
No.
Size
Price
Universal
Methylated Human DNA Standard
Methylated Human DNA standard for use with Zymo Research’s
EZ DNA Methylation™, EZ DNA Methylation-Gold™,
and EZ DNA Methylation-Direct™ Kits.
Universal
Methylated DNA Standard
Methylated DNA standard for use with Zymo Research’s
EZ DNA Methylation, EZ DNA Methylation-Gold, and EZ
DNA Methylation-Direct Kits.
Universal
Methylated Mouse DNA Standard
Methylated Mouse DNA standard for use with Zymo Research’s
EZ DNA Methylation, EZ DNA Methylation-Gold, and EZ
DNA Methylation-Direct Kits.
