|
ZR-96
DNA Clean & Concentrator™-5
• |
Simple, rapid recovery of ultra-pure DNA from PCR, endonuclease
digestions, “crude” DNA preps., etc. Perfect
for post-RT cDNA clean-up and purification of sequencing-ready
DNA from M13 phage infected culture supernatants. |
| • |
Column
and deep-well filtration plate designs allow DNA to be
eluted at high concentrations into minimal volumes of
water or
TE buffer. |
| • |
Eluted
DNA is well suited for use in PCR, DNA sequencing, DNA
ligation, endonuclease digestion, RNA transcription, radiolabeling,
etc.
|
|
|
|
SPECIFICATIONS
|
• |
Formats: Filtration Plate. |
| • |
DNA
Purity: Highly purified DNA is eluted with water and
is especially well suited for sequencing, ligation reactions,
and restriction endonuclease digestions. |
| • |
DNA
Size Limits: From 50 bp to 23 kb. |
| • |
DNA
Recovery: Typically, ≤ 5 µg total DNA can
be eluted with ≥ 6 µl water or low salt elution
buffer (≥ 10 µl for ZR-96 DCC™-5). For DNA
50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb
the recovery is 50-70%. |
| • |
Sample
Sources: DNA from PCR, restriction endonuclease digestions,
“crude” DNA preparations, etc. |
| • |
Product
Detergent Tolerance: ≤ 5% Triton X-100, ≤
5% Tween-20, ≤ 5% Sarkosyl, ≤ 0.1% SDS. |
| • |
Equipment:
Microcentrifuge (DCC™-5) or centrifuge w/ microplate carriers
(ZR-96 DCC™-5). |
|

DESCRIPTION
Zymo Research’s ZR-96 DNA Clean &
Concentrator-5™ (ZR-96 DCC™) provides
a hassle-free method for the rapid, large-scale (96 sample)
purification and concentration of high-quality DNA from
PCR samples, endonuclease digestions, or “crude”
plasmid preparations. Simply add the specially formulated
DNA Binding Buffer to your samples and
transfer to the wells of the supplied Zymo-Spin
I-96 Plate™. There is no need for organic
denaturants or chloroform. Instead, the product features
Fast-Spin column technology to yield high-quality, purified
DNA in just minutes. DNA purified using the ZR-96
DCC™ is suitable for nucleotide sequencing,
array analysis, PCR, nucleotide blotting, and restriction
endonuclease digestion procedures.
The ZR-96 DCC™ employs a single-buffer
system that allows for efficient DNA adsorption of up to
96 samples onto the matrix of the supplied Zymo-Spin
I-96 Plate™. The DNA is washed twice then
eluted with a small volume of water into a 96-well
Elution Plate. The entire DNA purification/concentration
procedure typically takes about 20 minutes. The figure below
shows a schematic highlighting the Zymo- Spin I-96
Plate™ design.
|

|
ORDERING INFORMATION:
| Products |
Protocol |
Cat
No. |
Size |
Price |
|
ZR-96
DNA Clean & Concentrator™-5
|
|
D4023 |
2x96
preps. |
$180.00 |
|
| ZR-96
DNA Clean & Concentrator™-5 |
|
D4024 |
4x96
preps. |
$350.00 |
|
|
| Components
Available Separately |
| DNA
Binding Buffer |
D4004-1-L |
100
ml |
$49.00 |
|
| DNA
Wash Buffer (concentrate) |
D4003-2-24 |
24
ml |
$28.00 |
|
| D4003-2-48 |
48
ml |
$52.00 |
|
| Zymo-Spin
I-96 Filtration Plates |
C2004 |
2
plates |
$124.00 |
|
|
Collection Plates |
C2002 |
2
plates |
$18.00 |
|
| ZR-96
Elution Plates |
C2003 |
2
plates |
$15.00 |
|
|
|
RELATED PRODUCTS:
|
|
|
|