Zymo Research - ZR-96 DNA Sequencing Clean-Up Kit™

ZR-96 DNA Sequencing Kit™ (Free Kit Offer)

HIGHLIGHTS

•	Simple 10 minute Bind, Wash, Elute procedure.
•	Flexible 15-20 µl elution volumes allow for direct loading of samples with no precipitation or drying steps.
•	Complete elimination of “dye blobs” with high quality Phred scores and long read lengths.
•	Reusable 96-well filtration plates that support both gel and capillary electrophoresis platforms.

FREE Full Sized Sample Kit Available

SPECIFICATIONS

•	Formats: Filtration Plate.
•	Sample Sources: Dye terminator sequencing reactions: Big Dye Terminator and other fluorescent based sequencing reactions.
•	DNA Purity: High quality, sequencing-ready DNA products that are free of unincorporated dye terminators, dNTPs, enzymes, and residual salts.
•	Equipment: Centrifuge with microplate carriers.

DESCRIPTION

The ZR-96 DNA Sequencing Clean-up Kit™ provides a simple method for the rapid removal of post-cycle sequencing reaction contaminants (i.e., unincorporated fluorescent dyes, residual salts, dNTPs, primers, and enzymes) from DNA extension products. These contaminants can often interfere with the quality and signal strength of sequencing data. In particular, unincorporated dyes can result in dye peaks (“dye blobs”) which may obscure portions of the sequencing chromatogram and interfere with base-calling accuracy of sequencing analysis software.

The ZR-96 DNA Sequencing Clean-up Kit™ employs a single-buffer system that allows for efficient DNA adsorption onto the matrix of the supplied Zymo-Spin™ IB-96 Plate. The DNA is washed then eluted with a small volume of water or loading dye containing formamide. The entire DNA purification procedure typically takes about 10 minutes.


Figure 1. Diagram of the ZR DNA Sequencing Clean-up procedure		Figure 2. Sequencing chromatogram of pGEM® DNA generated using an ABI 3730xl DNA analyzer. DNA was labeled with ABI BigDye v3.1 terminators and cleaned using the ZR DNA Sequencing Clean-up Kit™.

ORDERING INFORMATION:

Products	Description	Protocol	Cat No.	Size	Price
ZR-96 DNA Sequencing Clean-up Kit™	Rapid removal of post-cycle sequencing reaction contaminants from DNA.	PDF	D4052	(2) 96-well plates	$132.00
ZR-96 DNA Sequencing Clean-up Kit™		PDF	D4053	(4) 96-well plates	$272.00
ZR DNA Sequencing Clean-up Kit™	Rapid removal of post-cycle sequencing reaction contaminants from DNA.	PDF	D4050	50 preps.	$82.00
ZR DNA Sequencing Clean-up Kit™		PDF	D4051	200 preps.	$241.00
ZR-96 DNA Sequencing Clean-up Sample Kit™*	Full size sample kit.	PDF	D4052S	(2) 96-well plates	FREE
ZR DNA Sequencing Clean-up Sample Kit™*	Full size sample kit.	PDF	D4050S	50 preps.	FREE

*Limit one 50 prep or 2x96-well plates kit per US customer only.

Components Available Separately
Sequencing Binding Buffer	D4050-1-14	14 ml	$32.00
Sequencing Binding Buffer	D4050-1-55	55 ml	$82.00
Sequencing Wash Buffer	D4050-2-20	20 ml	$28.00
Sequencing Wash Buffer	D4050-2-70	70 ml	$45.00
Zymo-Spin IB-96 Plate	C2006	2 plates	$112.00
Collection Plates	C2002	2 plates	$18.00
96-Well PCR Plate	C2008	2 plates	$4.00

Download Data Sheet

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