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ZR-96 Zymoclean Gel DNA Recovery Kit

 HIGHLIGHTS


Quick, large-scale (96-well plate) recovery of pure DNA from TAE/TBE-buffered agarose gels.
The unique Zymo-Spin™ I-96 plate facilitates binding up to 5 µg DNA/well and elution with as little as 15 µl/well.
• Isolated DNA is ideal for DNA ligation, sequencing, DNA labeling, PCR, etc.


SPECIFICATIONS  

Sample Sources: DNA excised from TAE/TBE-buffered agarose gels.
DNA Purity: High quality, purified DNA is eluted with water which is especially well suited for sequencing, ligation reactions, andrestriction endonuclease digestions.
DNA Size Limits: From 75 bp to 23 kb.
DNA Recovery: Typically, up to 5 µg total DNA/well can be eluted with ≥ 15 µl water. For DNA 75 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.
DNA Quantity: Typically, up to 5 µg total DNA per column or well. For 50 bp – 10 kb: 70-90% 11 – 23 kb: 50-70%.
Product Detergent Tolerance: ≤ 5% Triton X-100, =5% Tween-20, ≤ 5% Sarkosyl, ≤ 0.1% SDS.
Equipment: Microcentrifuge, vortex, centrifuge w/ microplate carriers



DESCRIPTION

The Zymoclean™ Gel DNA Recovery Kit and ZR-96 Zymoclean™ Gel DNA Recovery Kit provide simple, rapid methods for the purification and concentration of high quality DNA from agarose gel slices. Both products feature a single buffer system that allows for efficient dissolving of agarose gel slices and the subsequent adsorption of DNA onto the matrices of the supplied Zymo-Spin I™ Columns or Zymo-Spin I-96™ Filtration Plate. The DNA is washed twice then eluted with a small volume of water. The entire DNA purification/concentration procedure typically takes about 15-20 minutes. The procedure is easy: simply add the specially formulated Agarose Dissolving Buffer (ADB™) to the gel slice containing your DNA sample, let dissolve, and transfer the mixture to the supplied column or filtration plate, then spin, wash, and elute the DNA. There is no need for organic denaturants or chloroform. Instead, the products utilize unique column and filtration plate technologies to yield high quality, purified DNA in just minutes. DNA purified using the Zymoclean™ Gel DNA Recovery Kit and ZR-96 Zymoclean™ Gel DNA Recovery Kit is perfectly suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc.



ORDERING INFORMATION:


Products
Protocol
Cat No.
Size
Price  
ZR-96 Zymoclean™ Gel DNA Recovery Kit
For 2x96 DNA clean-up reactions.
D4021
1 kit
$180.00
Add to Cart
ZR-96 Zymoclean™ Gel DNA Recovery Kit
For 4x96 DNA clean-up reactions.
D4022
1 kit
$350.00
Add to Cart

Components Available Separately
ADB-Buffer™ (Agarose Dissolving Buffer) D4001-1-50 50 ml $28.00
Add to Cart
D4001-1-100 100 ml $56.00
Add to Cart
DNA Wash Buffer (concentrate) D4003-2-24 24 ml $28.00
Add to Cart
D4003-2-48 48 ml $52.00
Add to Cart
Zymo-Spin™ I-96 Plates C2004 2 plates $124.00
Add to Cart
Elution Plates C2003 2 plates $15.00
Add to Cart
Collection Plates C2002 2 plates $18.00
Add to Cart

 







Zymoclean Spec Sheet - PDF
Download Spec Sheet

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