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ZR-96
Zymoclean™
Gel DNA Recovery Kit
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Quick, large-scale (96-well plate) recovery of pure DNA
from TAE/TBE-buffered agarose gels. |
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The
unique Zymo-Spin™ I-96 plate facilitates binding
up to 5 µg DNA/well and elution with as little as
15 µl/well. |
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Isolated DNA is ideal for DNA ligation, sequencing, DNA
labeling, PCR, etc.
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SPECIFICATIONS
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Sample Sources: DNA excised from TAE/TBE-buffered
agarose gels. |
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DNA
Purity: High quality, purified DNA is eluted with water
which is especially well suited for sequencing, ligation reactions,
andrestriction endonuclease digestions. |
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DNA Size Limits: From 75 bp to 23 kb. |
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DNA Recovery: Typically, up to 5 µg total
DNA/well can be eluted with ≥ 15 µl water. For DNA
75 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb
the recovery is 50-70%. |
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DNA
Quantity: Typically, up to 5 µg total DNA per
column or well. For 50 bp – 10 kb: 70-90% 11 – 23
kb: 50-70%. |
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Product
Detergent Tolerance: ≤ 5% Triton X-100, =5% Tween-20,
≤ 5% Sarkosyl, ≤ 0.1% SDS. |
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Equipment:
Microcentrifuge, vortex, centrifuge w/ microplate carriers |
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| DESCRIPTION
The Zymoclean™ Gel DNA Recovery Kit
and ZR-96 Zymoclean™ Gel DNA Recovery Kit
provide simple, rapid methods for the purification and concentration
of high quality DNA from agarose gel slices. Both products
feature a single buffer system that allows for efficient
dissolving of agarose gel slices and the subsequent adsorption
of DNA onto the matrices of the supplied Zymo-Spin I™
Columns or Zymo-Spin I-96™ Filtration Plate. The DNA
is washed twice then eluted with a small volume of water.
The entire DNA purification/concentration procedure typically
takes about 15-20 minutes. The procedure is easy: simply
add the specially formulated Agarose Dissolving Buffer (ADB™)
to the gel slice containing your DNA sample, let dissolve,
and transfer the mixture to the supplied column or filtration
plate, then spin, wash, and elute the DNA. There is no need
for organic denaturants or chloroform. Instead, the products
utilize unique column and filtration plate technologies
to yield high quality, purified DNA in just minutes. DNA
purified using the Zymoclean™ Gel DNA Recovery Kit
and ZR-96 Zymoclean™ Gel DNA Recovery Kit is perfectly
suited for use in DNA ligation reactions, sequencing, DNA
labeling reactions, PCR, etc. |

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ORDERING INFORMATION:
| Products |
Protocol |
Cat
No. |
Size |
Price |
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ZR-96
Zymoclean™ Gel DNA Recovery Kit
For 2x96 DNA clean-up reactions.
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D4021 |
1
kit |
$180.00 |
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ZR-96
Zymoclean™ Gel DNA Recovery Kit
For 4x96 DNA clean-up reactions.
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D4022 |
1
kit |
$350.00 |
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| Components
Available Separately |
| ADB-Buffer™
(Agarose Dissolving Buffer) |
D4001-1-50 |
50
ml |
$28.00 |
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| D4001-1-100 |
100
ml |
$56.00 |
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| DNA
Wash Buffer (concentrate) |
D4003-2-24 |
24
ml |
$28.00 |
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| D4003-2-48 |
48
ml |
$52.00 |
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| Zymo-Spin™
I-96 Plates |
C2004 |
2
plates |
$124.00 |
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Elution Plates |
C2003 |
2
plates |
$15.00 |
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| Collection
Plates |
C2002 |
2
plates |
$18.00 |
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