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ZR
Genomic DNA II Kit™
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Easy purification of genomic DNA from whole blood,
plasma, serum, body fluids, buffy
coat, lymphocytes, tissue, swabs
or cultured cells in less than 10 minutes using
innovative Fast-Spin column technology. |
| • |
DNA
isolated using this kit is ideal for PCR, Southern blotting,
methylation detection, sequencing, genotyping, etc. |
| • |
Compatible
with commonly used anticoagulants (i.e., EDTA, heparin,
citrate). |
| • |
Omits
the use of organic denaturants as well as proteinases.
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SPECIFICATIONS
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Sample Sources: Whole blood, plasma, or serum
from humans, mice, rats, etc. Also, tissue, cells from culture,
buccal cells, as well as a variety of liquid samples are effectively
processed using this kit. |
| • |
DNA
Purity: High-quality DNA is eluted with DNA Elution
Buffer or water that is especially well suited for PCR and other
downstream applications. Typical absorption indices are Ab260/280nm
> 1.8. |
| • |
DNA
Size Limits: Capable of recovering genomic DNA >
40 kb. In most instances, mitochondrial DNA is also recovered. |
| • |
DNA
Recovery: Typically, up to 25 µg total genomic
DNA is eluted into ≥ 50 µl DNA Elution Buffer or
water. Human whole blood will yield 33 ng - 66 ng DNA per µl
blood sampled. |
| • |
Product
Detergent Tolerance: ≤ 5% Triton X-100, ≤
5% Tween-20, ≤ 5% Sarkosyl, ≤ 0.1% SDS. |
| • |
Equipment:
microcentrifuge, vortex. |
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DESCRIPTION
The ZR Genomic DNA II Kit™
is a simple column-based procedure for the rapid isolation
of genomic DNA from a variety of biological sample sources.
The product has been optimized for maximal recovery of ultra-pure
DNA without RNA contamination and is compatible with whole
blood (fresh or stored), buffy coat, solid tissue, bone
marrow and buccal cells, cells from culture, and many biological
liquid samples. For processing, simply add the specially
formulated Genomic Lysis Buffer to your
sample and transfer the mixture to the supplied Zymo-Spin™
Column, then spin, wash, and elute the DNA. There
is no need for organic denaturants or chloroform. Instead,
the product features Fast-Spin column technology to yield
high quality, purified DNA in just minutes. DNA purified
using the ZR Genomic DNA II Kit™
is suitable for PCR, nucleotide blotting, DNA sequencing,
restriction endonuclease digestion, methylation analysis,
and other downstream applications. |
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ORDERING INFORMATION:
| Products |
Protocol |
Cat
No. |
Size |
Price |
|
ZR
Genomic DNA II Kit™
(Uncapped spin column format)
Reagents provided are for 50 preps.
|
|
D3006 |
1
kit |
$72.00 |
|
ZR
Genomic DNA II Kit™
(Uncapped spin column format)
Reagents provided are for 200 preps.
|
|
D3007 |
1
kit |
$250.00 |
|
ZR
Genomic DNA II Kit™
(Capped spin column format)
Reagents provided are for 50 preps. |
|
D3024 |
1
kit |
$81.00 |
|
ZR
Genomic DNA II Kit™
(Capped spin column format)
Reagents provided are for 200 preps. |
|
D3025 |
1
kit |
$263.00 |
|
|
| Components
Available Separately |
| Genomic
Lysis Buffer |
D3004-1-50 |
50
ml |
$30.00 |
|
| D3004-1-100 |
100
ml |
$52.00 |
|
| DNA
Pre-Wash Buffer |
D3004-5-15 |
15
ml |
$8.00 |
|
| D3004-5-30 |
30
ml |
$17.80 |
|
| D3004-5-50 |
50
ml |
$22.00 |
|
| g-DNA
Wash Buffer |
D3004-2-50 |
50
ml |
$15.00 |
|
| D3004-2-100 |
100
ml |
$26.00 |
|
| Zymobeads |
D3004-3-1 |
1
ml |
$20.00 |
|
| D3004-3-4 |
4
ml |
$75.00 |
|
| DNA
Elution Buffer |
D3004-4-4 |
4
ml |
$6.00 |
|
| D3004-4-16 |
16
ml |
$16.00 |
|
| Zymo-Spin
IIN Columns (uncapped) |
C1019-50 |
50
pk |
$35.00 |
|
| C1019-250 |
250
pk |
$152.00 |
|
| Zymo-Spin
IIC Columns |
C1011-50 |
50
pk |
$45.00 |
|
| C1011-250 |
250
pk |
$162.00 |
|
| Collection
Tubes |
C1001-50 |
50
pk |
$5.00 |
|
| C1001-500 |
500
pk |
$30.00 |
|
| C1001-1000 |
1,000
pk |
$50.00 |
|
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RELATED PRODUCTS:
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The
ZR Genomic DNA II Kit™ effectively isolates genomic
DNA from a variety of sample sources (Indicated at top of figure).
Samples were processed in duplicate with equivalent volumes of
eluted DNA loaded into each lane of a 0.8% w/v agarose/EtBr/TAE
gel. |
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