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ZR
Insect/Tissue DNA Kit™-25
(Free
Sample Kit Available)
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• Simple method for the isolation of up to 25
µg DNA from fresh, frozen or stored insect and
arthropod specimens in as little as 15 minutes. |
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The
organic denaturant/proteinase K-free procedure
is ideal for processing small organisms including mosquitoes,
bees, lice, ticks, and D. melanogaster. |
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State-of-the-art,
ultra-high density BashingBeads™
are fracture resistant, chemically inert and can be used
with any bead mill, disrupter, or vortex that can
accommodate standard 2 ml tubes. |
| • |
Compatible
with mammalian tissues, cultured cells, and whole blood.
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SPECIFICATIONS
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• |
Formats: Bead Beating, Spin Column. |
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Sample
Sources: Samples (n ≥ 1 and ≤ 50 mg) of
fresh, frozen, or stored insects including: mosquitoes, bees,
lice, ticks, D. melanogaster, etc. Also, compatible
with fresh or frozen mammalian tissues (e.g., soft tissues like
liver and brain) as well as cultured cells, and whole blood. |
| • |
DNA
Yield: Expected yields can range from 1-5 µg
DNA per mg specimen sampled. For mammalian tissues, yields are
1-3 µg DNA per mg of skeletal, heart and brain tissues
and 3-5 µg DNA per mg of liver, kidney, and lung tissues.
Whole blood will yield from 3-7 µg DNA per 100 µl. |
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DNA
Purity: High quality DNA is eluted with DNA
Elution Buffer that is well suited for PCR amplification,
endonuclease digestion, etc. A260/A280
> 1.8 |
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DNA
Size Limits: > 1 kb |
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DNA
Recovery: Typically, up to 25 µg
total DNA is eluted into ≥ 25 µl DNA Elution
Buffer per sample. |
| • |
Equipment:
Microcentrifuge, vortex, cell disrupter/pulverizer (optional). |
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| DESCRIPTION
The ZR Insect/Tissue DNA Kit™
is designed for the simple, rapid isolation of total DNA
(e.g., genomic, viral, mitochondrial) from small amounts
of fresh, frozen or stored insect specimens including mosquitoes,
bees, lice, ticks, and D. melanogaster. The procedure
is easy and can be completed in as little as 15 minutes:
samples are added directly to a ZR BashingBead™
Lysis Tube and rapidly and efficiently lysed without
using organic denaturants or proteinases. The DNA is isolated
and purified using our Fast-Spin column technology and is
ideal for downstream molecular-based applications including
PCR, array, genotyping, etc. PCR inhibitors are effectively
removed during the purification process. Also, the procedure
is compatible with mammalian tissues, whole blood, and cultured
cells. A schematic of the ZR Insect/Tissue DNA Kit™
procedure is shown below.
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ORDERING INFORMATION:
| Products |
Description |
Protocol |
Cat
No. |
Size |
Price |
|
ZR
Insect/Tissue
DNA Kit-™-25 |
50
DNA preps. Up to 25 µg DNA per prep |
|
D6016 |
1
kit |
$132.00 |
|
ZR Insect/Tissue
DNA Kit-™-5 |
50
DNA preps. Up to 5 µg DNA per prep. |
|
D6015 |
1
kit |
$132.00 |
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*Free
kit offer valid online only and while supplies last.
Limit 1 per U.S. customer only. |
| Components
Available Separately |
| ZR
Bashing Bead Lysis Tubes |
S6002-50 |
50
tubes |
$92.00 |
|
| Lysis
Solution |
D6001-3-40 |
40
ml |
$25.00 |
|
| Genomic
Lysis Buffer |
D3004-1-100 |
100
ml |
$52.00 |
|
| DNA
Pre-Wash Buffer |
D3004-5-15 |
15
ml |
$8.00 |
|
| g-DNA
Wash Buffer |
D3004-2-50 |
50
ml |
$15.00 |
|
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DNA Elution Buffer |
D3004-4-10 |
10
ml |
$12.00 |
|
| Zymo-Spin
IV Filters
(Orange Tops) |
C1007-50 |
50
filters |
$50.00 |
|
| Zymo-Spin
IC Columns (capped) |
C1004-50 |
50
columns |
$46.00 |
|
| C1004-250 |
250
columns |
$197.00 |
|
| Collection
Tubes |
C1001-50 |
50
tubes |
$5.00 |
|
| C1001-500 |
500
tubes |
$30.00 |
|
| C1001-1000 |
1,000
tubes |
$50.00 |
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| Lysis
Instruments Available Separately |
| Disruptor
Genie™ (120V w/ 2 ml tube holder assembly) |
S6001-120 |
1
unit |
$425.00 |
|
| Disruptor
Genie™ (240V w/ 2 ml tube holder assembly) |
S6001-240 |
1
unit |
$425.00 |
|
TurboMix
Attachment, 2.0 ml
Permanently mounts to most existing Vortex-Genie
2 and Vortex-Genie 2T mixers converting them to a Disruptor
Genie™. |
S6004-2 |
1
unit |
$172.00 |
|
| FastPrep®-24
Instrument |
S6005 |
1
unit |
$7,625.00 |
 |
HiPrep™
Attachment
(Accomodates
48 x 2 ml tubes) |
S6005-1 |
1
unit |
$1,200.00 |
|
CryoPrep™
Attachment
(Accomodates
24 x 2 ml tubes) |
S6005-2 |
1
unit |
$1,550.00 |
|
TeenPrep™
Attachment
(Accomodates
12x 15 ml tubes) |
S6005-3 |
1
unit |
$1,200.00 |
|
BigPrep™
Attachment
(Accomodates
2 x 50 ml tubes)
|
S6005-4 |
1
unit |
$1,200.00 |
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