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Z-Competent™
E. coli Strains - HB101
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Feature
fast transformation kinetics: No heat shock, no lengthy
incubations, no outgrowth procedures, no wait! |
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High
transformation efficiencies: 108-109
transformants/µg plasmid DNA. |
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Simple:
Mix DNA with cells for a few seconds and plate. Mix &
Go!
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SPECIFICATIONS
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Genotype: F-∆(gpt-proA)62 leuB6 supE44
ara-14 galkK2 lacY1 ∆(mcrC-mrr) rpsL20 (Strr
) xyl-5 mtl-1 recA13(4) |
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Total
Processing time: 10-20 minutes. |
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Availability:
Either as 10 tubes (each containing a 100 µl aliquot of
Z-Competent™ cells), or as a 96-well plate (w/ 12 removable
8-well strips) containing 50 µl aliquots of Z-Competent™
in each well. |
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Storage:
-70°C. |
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| DESCRIPTION
Z-Competent™
E. coli
are premade chemically competent cells used for simple and
highly efficient DNA transformation. Z-Competent™
E. coli cells are made chemically competent by
a unique method using ZymoBroth™
that completely eliminates the need for heat shock and related
procedures. For transformation, DNA can be added directly
to Z-Competent™ cells and the mixture spread directly
to a culture plate - Mix & Go! Transformation efficiencies
typically range from 108-109 transformants/µg
of pUC19 DNA) (see figures below), which make the cells
optimal for cloning, sub-cloning, PCR fragment cloning,
library construction, etc. Premade Z Competent™ cells
are supplied as a pack of 10 convenient 100 µl/tube
aliquots or in a 96-well format (12x8-well strips) of 50
µl/tube. |
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Z-Competent™
E. coli cells prepared with
ZymoBroth™ display
fast transformation kinetics and high transformation
efficiencies. Figures above show the transformation
kinetics for Z-Competent™ JM109 and
BL21 strains of E. coli generated using ZymoBroth™
and SOB growth media. Plasmid DNA (pUC19)
was used for transformation and the data are
the averages of three individual experiments. |
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ORDERING INFORMATION:
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Enter
Promo Code: ZB406 upon checkout. US customers only. |
| Products |
Protocol |
Cat
No. |
Size |
Price |
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JM109
|
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T3003
|
10 x 100 µl aliquots (10 tubes) |
$110.00 |
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| T3005 |
96 x 50 µl aliquots (1 96-well plate) |
$420.00 |
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DH5α |
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T3007 |
10 x 100 µl aliquots (10 tubes) |
$110.00 |
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| T3009 |
96 x 50 µl aliquots (1 96-well plate) |
$420.00 |
|
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HB101 |
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T3011 |
10
x 100 µl aliquots (10 tubes) |
$110.00 |
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| T3013 |
96 x 50 µl aliquots (1 96-well plate) |
$420.00 |
|
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C600 |
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T3015 |
10 x 100 µl aliquots (10 tubes) |
$110.00 |
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| TG1
|
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T3017 |
10
x 100 µl aliquots (10 tubes) |
$110.00 |
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