| DESCRIPTION
Z-Competent™
E. coli
are premade chemically competent cells used for simple and
highly efficient DNA transformation. Z-Competent™
E. coli cells are made chemically competent by
a unique method using ZymoBroth™
that completely eliminates the need for heat shock and related
procedures. For transformation, DNA can be added directly
to Z-Competent™ cells and the mixture spread directly
to a culture plate - Mix & Go! Transformation efficiencies
typically range from 108-109 transformants/µg
of pUC19 DNA) (see figures below), which make the cells
optimal for cloning, sub-cloning, PCR fragment cloning,
library construction, etc. Premade Z Competent™ cells
are supplied as a pack of 10 convenient 100 µl/tube
aliquots or in a 96-well format (12x8-well strips) of 50
µl/tube. |
