Zymo Research - Z Competent Transformation Buffer Set

Z-Competent™ E. coli Transformation Buffer Set

HIGHLIGHTS

•	Simple method for generating Z-Competent™ E. coli for rapid, reliable and highly efficient DNA transformation (>10⁸ transformants/µg plasmid DNA).
•	Straightforward procedure: grow, wash, and then resuspend cells.
•	The Z-Competent™ E. coli Transformation Kit (T3001) features a specially formulated ZymoBroth™ growth medium that dramatically increases E. coli transformation efficiency, typically on the order of 5 to 100-fold for most lab strains.
•	Z-Competent™ cells can be transformed in seconds (Mix & Go!) without heat shocking, lengthy incubations, or outgrowth steps.

DESCRIPTION

The Z-Competent™ E. coli Transformation Kit & Buffer Set are simple methods for generating Z-Competent™ E. coli for rapid, reliable and highly efficient DNA transformation. The methods eliminate the requirement of heat-shocking and related procedures. Instead, transformation can be performed by adding DNA to Z-Competent™ cells and the mixture spread directly to a culture plate - Mix & Go! Transformation efficiencies typically range from 10⁸-10⁹ transformants/µg of pUC19 DNA, but can vary depending on the strain of E. coli. Most E. coli strains respond well to the Z-Competent™ method and demonstrate fast transformation kinetics (see figures below).

ZymoBroth™ medium dramatically increases the transformation efficiencies of a broad range of E. coli strains. The figure above shows the relative transformation efficiencies for different E. coli strains generated using SOB and ZymoBroth™. Generally, ZymoBroth™ medium enhances E. coli transformation efficiencies better for difficult to transform strains.

ORDERING INFORMATION:

Enter Promo Code: ZB406 upon checkout. US customers only.

Products	Protocol	Cat No.	Size	Price
*Z-Competent E. coli* Transformation Kit™** (E. coli growth medium ZymoBroth™ is supplied).	PDF	T3001	1 kit	$92.00
*Z-Competent E. coli* Transformation Buffer Set™** (E. coli growth medium, ZymoBroth™, is NOT supplied).	PDF	T3002	1 kit	$99.00

Components Available Separately
ZymoBroth	M3015-100	100 ml	$16.00
ZymoBroth	M3015-500	500 ml	$51.00
Wash Buffer (2X Stock)	T3001-2-10	10 ml	$24.00
Wash Buffer (2X Stock)	T3001-2-30	30 ml	$45.00
Competent Buffer (2X Stock)	T3001-3-10	10 ml	$24.00
Competent Buffer (2X Stock)	T3001-3-30	30 ml	$45.00
Dilution Buffer	T3001-4-20	20 ml	$10.00
Dilution Buffer	T3001-4-60	60 ml	$22.00