Zymo Research - ZymoBroth™

DESCRIPTION

ZymoBroth™ (ZB™) is a specially formulated E. coli growth medium used for the preparation of highly competent E. coli cells for chemical transformation. When compared to classic SOB growth medium, competent E. coli cells prepared with cells prepared with ZymoBroth™ can dramatically increase transformation efficiency, typically on the order of 5-100 fold (depending on the E. coli strain). When used in conjunction with our strain). When used in conjunction with our popular Z-Competent™ E. coli Transformation Buffer System, ZB™ medium will enable the researcher to generate\ their own "home-made" super Z- Competent™ E. coli cells for chemical transformation purposes. cells for chemical transformation purposes. ZB™ medium has been tested on a wide range of E. coli strains. Our accumulated data indicate that ZB™ medium can stimulate the transformation effi ciency of all E. coli strains tested, including K12 derivatives (Such as JM109, HB101, etc) and B strain derivatives (such as BL21, etc). The figure below strains tested, including K12 derivatives (Such as JM109, HB101, etc) and B strain derivatives (such as BL21, etc). The figure below shows the relative transformation efficiencies for different E. coli strains when using SOB and strains when using SOB and ZymoBroth™. Generally, the ZB™ medium enhances E. coli transformation efficiency better for strains that are difficult to transform than for those that are easy to transform.