Zymo Research - DNA Free RNA Kit

DNA Free RNA Kit™

HIGHLIGHTS

•	Convenient method for DNA-free RNA preparation.
•	Column design allows RNA to be eluted at high concentrations into minimal volumes of RNase-free water in just minutes.
•	Purified RNA is suitable for use in RNA-based procedures including RT- PCR.
•	Omits the use of organic denaturants and proteinases.

SPECIFICATIONS

•	RNA Purity: High quality, purified RNA is eluted into RNase-free water and is suitable for RNA-based manipulations including RT-PCR.
•	RNA Recovery: Typically, RNA is eluted into as little as 6-10 µl RNase-free water allowing for a highly concentrated sample. The RNA binding capacity of the supplied column is 5 µg.
•	RNA Storage: Recommended that 1 U/10 µl RNase inhibitor be added to the RNA prior to storage at -70°C.
•	Sample Sources: RNA preparations from DNA.
•	Stability of Product Reagents: Integrity of kit components is guaranteed for up to one year from date of purchase.
•	Quality Control : Reagents are routinely tested on a lot-to-lot basis to ensure they provide maximal performance and reliability.

DESCRIPTION Zymo Research’s DNA-Free RNA Kit™ provides a hassle-free method for the rapid preparation of DNA-free, high quality RNA for manipulations such as RT-PCR. The procedure includes the use of certified RNase-free DNase followed by Fast-Spin column technology to yield purified RNA in small volumes of RNase-free water in just minutes. The result is highly-concentrated, DNA-free RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.
	DNA-free RNA is effectively recovered following treatment using the DNA-Free RNA Kit™. RNA samples (a and b) were treated to eliminate trace amounts of DNA. Over 90% of theRNA was recovered as demonstrated by a 1% w/v agarose gel.

ORDERING INFORMATION:

Products	Protocol	Cat No.	Size	Price
DNA-FREE RNA Kit ™ (capped) Reagents provided are for 50 preps.	PDF	R1013	1 kit	$142.00
DNA-FREE RNA Kit™ (capped) Reagents provided are for 200 preps.	PDF	R1014	1 kit	$510.00
DNA-FREE RNA Kit™ (uncapped) Reagents provided are for 50 preps.	PDF	R1027	1 kit	$142.00
DNA-FREE RNA Kit™ (uncapped) Reagents provided are for 200 preps.	PDF	R1028	1 kit	$510.00

Components Available Separately
RNA Binding Buffer	R1013-2-50	50 ml	$65.00
RNA Binding Buffer	R1013-2-100	100 ml	$112.00
RNA Wash Buffer	R1003-3-2.4	2.4 ml	$9.00
	R1003-3-6	6 ml	$15.00
	R1003-3-12	12 ml	$26.00
	R1003-3-24	24 ml	$52.00
	R1003-3-48	48 ml	$92.00
DNase I (100 U/µl) /10X DNase I Buffer	E1007	100 units	$27.00
Dnase/RNase Free water	W1001-1	1 ml	$2.00
	W1001-4	4 ml	$3.00
	W1001-6	6 ml	$4.00
	W1001-10	10 ml	$6.00
Zymo-Spin I™ Columns (uncapped)	C1003-50	50 columns	$35.00
Zymo-Spin I™ Columns (uncapped)	C1003-250	250 columns	$152.00
Zymo-Spin IC™ Columns (capped)	C1004-50	50 columns	$46.00
Zymo-Spin IC™ Columns (capped)	C1004-250	250 columns	$197.00
Collection Tubes	C1001-50	50 tubes	$5.00
	C1001-500	500 tubes	$30.00
	C1001-1000	1,000 tubes	$50.00