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Mini
RNA Isolation I Kit™
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Allows for the simple, rapid recovery of purified total
RNA from a wide range of samples using unique column and
filtration plate technologies. |
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Simply and reliably isolates total RNA from small sample
amounts (1x101-1x105 cells). |
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Column
design allows RNA to be eluted at high concentrations
into minimal volumes of solvent. |
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Eluted RNA is suitable for use in RNA-based procedures. |
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Omits the use of organic denaturants as well as proteinases.
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SPECIFICATIONS
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Formats: Spin Column (capped, uncapped)/Filtration
Plate. |
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RNA
Recovery: Typically, up to 5 µg RNA (per
sample) is eluted into ≥ 6 µl RNase-free water allowing
for a highly concentrated sample. Recovery is approximately
80-95% of input RNA. |
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RNA
Storage: Recommended that 1 U/10 µl RNase
inhibitor be added to the RNA prior to storage at -70°C. |
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RNA
Size Limits: From 200 bases
up to 35 kb. |
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Sample
Sources: 1x101-1x105
cells from culture, needle biopsy, or small amounts of solid
tissue. |
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Equipment:
Microcentrifuge (Mini RNA Isolation I Kit™) or centrifuge
w/ microplate carriers (ZR-96 Mini RNA Isolation I Kit™). |
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DESCRIPTION
Both the Mini RNA Isolation Kit™
and ZR-96 Mini RNA Isolation Kit™
provide hassle-free methods for the rapid purification of
high quality total RNA from small amounts of cells derived
from culture, needle biopsy, micro-dissected tissue, etc.
The procedures combine a unique, single step RNA extraction/binding
buffer with Fast-Spin column and filtration plate
technologies to yield high quality, purified RNA in less
than an hour. The Mini RNA Isolation Kit™ and ZR-96
Mini RNA Isolation Kit™ allow for the efficient recovery
of total RNA, from 1x101-1x105 cells.
The procedures are easy: simply add the provided RNA Extraction
Buffer to extract total RNA from cells of interest then
purify the RNA using the provided Zymo-Spin™ Columns
or Zymo-Spin I-96™ Plates. The result is highly concentrated,
purified RNA that is suitable for subsequent RNA-based analyses.
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ORDERING INFORMATION:
| Products |
Protocol |
Cat
No. |
Size |
Price |
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Mini
RNA Isolation I Kit™
For
50 preps.
(Supplied with uncapped columns)
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R1005 |
1
kit |
$118.00 |
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Mini
RNA Isolation I Kit™
For
50 preps.
(Supplied with capped columns) |
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R1006 |
1
kit |
$122.00 |
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Components
Available Separately |
| RNA
Extraction Buffer |
R1003-2-3 |
3
ml |
$26.00 |
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| R1003-2-12 |
12
ml |
$58.00 |
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| RNA
Wash Buffer |
R1003-3-2.4 |
2.4
ml |
$9.00 |
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| R1003-3-6 |
6
ml |
$15.00 |
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| R1003-3-12 |
12
ml |
$26.00 |
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| R1003-3-24 |
24
ml |
$52.00 |
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| R1003-3-48 |
48
ml |
$92.00 |
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| RNA
Elution Buffer |
R1003-4 |
1
ml |
$15.00 |
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| Zymo-Spin
I Columns (uncapped) |
C1003-50 |
50
columns |
$35.00 |
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| C1003-250 |
250
columns |
$152.00 |
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| Zymo-Spin
IC Columns (capped) |
C1004-50 |
50
columns |
$45.50 |
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| C1004-250 |
250
columns |
$197.00 |
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| Collection
Tubes |
C1001-50 |
50
tubes |
$5.00 |
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| C1001-500 |
500
tubes |
$30.00 |
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| C1001-1000 |
1,000
tubes |
$50.00 |
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