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Pinpoint Slide RNA Isolation System I

 HIGHLIGHTS


Allows for the isolation of total RNA from tissue mounted on glass slides.
Simple procedure combines Pinpoint™ tissue sampling technology with a onestep RNA extraction/purification method.
Isolates RNA that is suitable for use in RNA-based procedures including RT-PCR.
Omits the use of organic denaturants as well as proteinases.


SPECIFICATIONS

Format: Pinpoint™ Sampling, Spin Column.
RNA Recovery: Typically, up to 5 µg RNA is eluted into ≥ 6 µl elution buffer allowing for a highly concentrated sample. Recovery is approximately 80-95% of input RNA.
RNA Storage: Recommended that 1 U/10 µl RNase inhibitor be added to the RNA prior to storage at -70°C.
RNA Size Limits: From 200 bases up to 35 kb.
Sample Sources: Cells from tissue sections on glass slides.



DESCRIPTION

The Pinpoint™ Slide RNA Isolation System I is an innovative product designed by those at Zymo Research to isolate RNA from any targeted area of tissue on a microscopic slide. The system combines a powerful Pinpoint™ tissue sampling method with a unique, single-step RNA extraction/binding buffer that includes Fast-Spin column technology to yield high quality purified RNA. This product makes targeted tissue RNA isolation simple and quick. There is no use of organic solvents or other toxic reagents used in this method. There is also no need for expensive specialized equipment or computer software. This kit allows for the efficient recovery of RNA from fresh tissue sections for subsequent RNA analyses including RT-PCR.


ORDERING INFORMATION:


Products
Protocol
Cat No.
Size
Price  
Pinpoint™ Slide RNA Isolation System I
Reagents provided are for 50 preps.
R1003

1 kit

$141.00
Add to Cart

Components Available Separately
Pinpoint Solution D3001-1 1 ml $68.00
Add to Cart
RNA Extraction Buffer R1003-2-3 3 ml $26.00
Add to Cart
R1003-2-12 12 ml $58.00
Add to Cart
R1003-2-50 50 ml $212.00
Add to Cart
R1003-2-100 100 ml $482.00
Add to Cart
RNA Wash Buffer R1003-3-2.4 2.4 ml $9.00
Add to Cart
R1003-3-6 6 ml $15.00
Add to Cart
R1003-3-12 12 ml $26.00
Add to Cart
R1003-3-24 24 ml $52.00
Add to Cart
R1003-3-48 48 ml $92.00
Add to Cart
RNA Elution Buffer R1003-4 1 ml $15.00
Add to Cart
Zymo-Spin I Columns (uncapped) C1003-50 50 columns $35.00
Add to Cart
C1003-250 250 columns $152.00
Add to Cart
Collection Tubes C1001-50 50 tubes $5.00
Add to Cart
C1001-500 500 tubes $30.00
Add to Cart
C1001-1000 1,000 tubes $50.00
Add to Cart

RELATED PRODUCTS:

Products
Cat No.
Size
Price  
Pinpoint™ Slide RNA Isolation System II
Reagents provided are for 50 preps.
R1007

1 kit

$225.00
Add to Cart

 



Simply apply the Pinpoint™ Solution to a selected area of tissue on a glass slide. The solution will air-dry forming a thin film that embeds the tissue underneath. This is then lifted from the slide and transferred to a tube. Following treatment of the tissue with RNA Extraction Buffer, the extracted RNA is washed and concentrated using a Zymo-Spin I™ Column. The isolated RNA can then be used for analysis including RT-PCR.


RT-PCR of RNA recovered from human tissue using the Pinpoint™ RNA Isolation System. Amplicons (in duplicate) are from: A) a human ß-actin transcript; B) an arbitrary human transcript from chromosome 3.
RT-PCR of RNA recovered from human tissue using the Pinpoint™ RNA Isolation System. Amplicons (in duplicate) are from: A) a human ß-actin transcript; B) an arbitrary human transcript from chromosome 3.

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