|
Pinpoint™
Slide RNA Isolation System II
• |
Allows for the isolation of total RNA from paraffin-embedded
tissue on glass slides. |
| • |
Simple procedure combines Pinpoint™ tissue sampling
technology with a onestep
RNA extraction/purification method. |
| • |
Isolates
RNA that is suitable for use in RNA-based procedures including
RTPCR.
|
|
|
|
SPECIFICATIONS
| • |
RNA
Purity: Generally, traces of DNA are present
in the eluted RNA fraction. DNA-free RNA can be obtained with
subsequent use of Zymo Research’s DNA-Free RNA Kit™
(R1013, R1014, R1027, & R1028). |
| • |
RNA
Recovery: Typically, RNA is eluted into as
little as 6-10 µl elution buffer allowing for a highly
concentrated sample. The RNA binding capacity of the columns
is 5 µg. |
| • |
RNA
Storage: Recommended that 1 U/10 µl RNase
inhibitor be added to the RNA prior to storage at -70°C. |
| • |
Sample
Sources: Cells from paraffin-embedded tissue
sections on glass slides. |
| • |
Stability
of Product Reagents: Integrity of kit components is
guaranteed for up to one year from date of purchase. |
| • |
Quality
Controls: Reagents are routinely tested on a lot-to-lot
basis to ensure they provide maximal performance and reliability. |
|
DESCRIPTION
The Pinpoint™ Slide RNA Isolation System II
is an innovative product designed by those at Zymo Research
to isolate RNA from any targeted area of paraffin-embedded
tissue on a microscopic slide. The system combines a powerful
Pinpoint™ tissue sampling method with a unique, single-step
RNA extraction/binding buffer that includes Fast-Spin column
technology to yield high quality RNA. Unlike current UV-based
methods, this product makes isolation of paraffin-embedded
tissue RNA simple and quick. There is also no need for expensive
specialized equipment or computer software. This kit allows
for the efficient recovery of RNA from fresh tissue sections
for subsequent RNA analyses including RT-PCR. |
|
ORDERING INFORMATION:
| Products |
Protocol |
Cat
No. |
Size |
Price |
|
Pinpoint™
Slide RNA Isolation System II
Reagents provided are for 50 preps.
|
|
R1007 |
1
kit |
$225.00 |
|
|
| Components
Available Separately |
| Pinpoint
Solution |
D3001-1 |
1
ml |
$68.00 |
|
| Proteinase
K and Storage Buffer Set |
D3001-2 |
12
ml |
$58.00 |
|
| RNA
Digestion Buffer |
R1007-1 |
1.2
ml |
$26.00 |
|
| RNA
Extraction Buffer |
R1003-2-3 |
3
ml |
$26.00 |
|
| R1003-2-12 |
12
ml |
$58.00 |
|
| R1003-2-50 |
50
ml |
$212.00 |
|
| R1003-2-100 |
100
ml |
$482.00 |
|
| RNA
Wash Buffer |
R1003-3-2.4 |
2.4
ml |
$9.00 |
|
| R1003-3-6 |
6
ml |
$15.00 |
|
| R1003-3-12 |
12
ml |
$26.00 |
|
| R1003-3-24 |
24
ml |
$52.00 |
|
| R1003-3-48 |
48
ml |
$92.00 |
|
| RNA
Elution Buffer |
R1003-4 |
1
ml |
$15.00 |
|
| Zymo-Spin
I Columns (uncapped) |
C1003-50 |
50
columns |
$35.00 |
|
| C1003-250 |
250
columns |
$152.00 |
|
|
|
RELATED PRODUCTS:
| Products |
Cat
No. |
Size |
Price |
|
Pinpoint™
Slide RNA Isolation System I
Reagents provided are for 50 preps.
|
R1003 |
1
kit |
$141.00 |
|
|
|
|
|
| RT-PCR
of RNA recovered from human tissue using the Pinpoint™
RNA Isolation System. Amplicons (in duplicate) are from:
A) a human ß-actin transcript; B) an arbitrary human transcript
from chromosome 3. |
|
