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RNA
Clean-up Kit™ - 5
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Fast (2 minute) procedure. |
| • |
Column
design allows RNA to be eluted at high concentrations
into minimal volumes of RNase-free water in just minutes. |
| • |
Purified
RNA is suitable for use in RNA-based procedures including
RT- PCR. |
| • |
Omits
the use of organic denaturants and proteinases.
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SPECIFICATIONS
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• |
Format: Spin Column (capped, uncapped). |
| • |
RNA
Purity: High quality, purified RNA is eluted
with ≥ 6 µl RNase-free water and is suitable for
RNA-based manipulations including RT-PCR. |
| • |
RNA
Storage: Recommended that 1 U/10 µl RNase
inhibitor be added to the RNA prior to storage at -70°C. |
| • |
RNA
Size Limits: From 200 bases
up to 20 kb. |
| • |
Sample
Sources: RNA from DNase digestion, in vitro
transcription, etc. |
| • |
RNA
Recovery: Typically, RNA is eluted into >=
6 µl RNase-free water allowing for a highly concentrated
sample. Recovery is approximately 80-95% of input RNA. The RNA
binding capacity of the supplied column is 5 µg. |
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| DESCRIPTION
Zymo Research’s RNA Clean-up Kit-5™
provides a hassle-free method for the rapid preparation
of up to 5 µg (per prep.) of high-quality RNA for
manipulations such as RT-PCR. This simple procedure is based
on the use of a unique single-buffer system and Fast-Spin
column technology. The procedure is easy: just add the buffer
to your sample and the RNA is then cleaned and concentrated
into 6-10 µl of RNase-free water using a Zymo-Spin™
Column. RNA ranging in size from 50 bp-20 kb can be safely
treated and recovered using this kit. The result is highly-concentrated,
purified RNA that is suitable for subsequent RNA-based methods
including RT-PCR, hybridization, etc. |
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ORDERING INFORMATION:
| Products |
Protocol |
Cat
No. |
Size |
Price |
|
RNA
Clean-up Kit™-5
50 preps. (capped columns)
|
|
R1015 |
1
kit |
$122.00 |
|
RNA
Clean-up Kit™-5
200 preps. (capped columns) |
|
R1016 |
1
kit |
$420.00 |
|
RNA
Clean-up Kit™-5
50 preps. (uncapped columns) |
|
R1023 |
1
kit |
$122.00 |
|
RNA
Clean-up Kit™-5
200 preps. (uncapped columns) |
|
R1024 |
1
kit |
$420.00 |
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Components
Available Separately |
| RNA
Binding Buffer |
R1013-2-50 |
50
ml |
$65.00 |
|
| R1013-2-100 |
100
ml |
$112.00 |
|
| RNA
Wash Buffer (concentrate) |
R1003-3-2.4 |
2.4
ml |
$9.00 |
|
| R1003-3-6 |
6
ml |
$15.00 |
|
| R1003-3-12 |
12
ml |
$26.00 |
|
| R1003-3-24 |
24
ml |
$52.00 |
|
| R1003-3-48 |
48
ml |
$92.00 |
|
| Zymo-Spin
I Columns (uncapped) |
C1003-50 |
50
columns |
$35.00 |
|
| C1003-250 |
250
columns |
$152.00 |
|
| Zymo-Spin
IC Columns (capped) |
C1004-50 |
50
columns |
$46.00 |
|
| C1004-250 |
250
columns |
$197.00 |
|
| Collection
Tubes |
C1001-50 |
50
tubes |
$5.00 |
|
| C1001-500 |
500
tubes |
$30.00 |
|
| C1001-1000 |
1,000
tubes |
$50.00 |
|
| DNase/RNase
Free water |
W1001-1 |
1
ml |
$2.00 |
|
| W1001-4 |
4
ml |
$3.00 |
|
| W1001-6 |
6
ml |
$4.00 |
|
| W1001-10 |
10
ml |
$6.00 |
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| RNase-Free
Tubes 1.5 ml |
C2001-50 |
50
tubes |
$8.00 |
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