| DESCRIPTION
Zymo Research’s ZR-96 Viral
RNA Kit™ provides a hassle-free method for
the rapid, large scale (i.e., 96 sample) isolation of high-quality
viral RNA from a wide range of biological sources. The kit
can be used to successfully isolate viral RNA from cellfree
body fluids as well as cellular suspensions having concentrations
≤1x105 cells/ml. The kit has been rigorously
tested and used to isolate viral RNA from samples containing
enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza
A virus, flaviviruses, measles virus, parainfluenza virus,
and parvovirus (a ssDNA virus). The ZR-96 Viral RNA Kit™
employs a single-buffer system that allows for efficient
extraction and adsorption of RNA from up to 96 samples onto
the matrix of the supplied Zymo-Spin I-96 Plate™.
The RNA is washed twice then eluted with a small volume
of RNAse-Free Water into a 96-well Elution Plate. The entire
RNA isolation procedure typically takes about 15 minutes,
and the eluted viral RNA is suitable for use in various
RNA-based procedures including RT-PCR. The figures below
show a schematic highlighting the Zymo-Spin I-96 Plate™
design and results from RNA isolated using the ZR Viral
RNA Kit™, respectively.
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