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ZR
Whole-Blood Total RNA Kit™
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Convenient method for purifying total RNA from whole-blood
samples. |
| • |
Compatible
with commonly used anticoagulants (i.e., EDTA, heparin,
citrate). |
| • |
Allows
RNA to be eluted at high concentrations into minimal volumes
of RNase-free water in just minutes. |
| • |
Purified RNA is suitable for use in RNA-based procedures
including RT- PCR. |
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Omits
the use of organic denaturants and proteinases.
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SPECIFICATIONS
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Format: Spin Column. |
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RNA
Purity: High quality, purified RNA is eluted
into RNase-free water and is suitable for RNA-based manipulations
including RTPCR. |
| • |
RNA
Recovery: For the standard procedure, up to
25 µg RNA is eluted into 40 µl RNase-free water. |
| • |
RNA
Storage: Recommended that 1 U/10 µl RNase
inhibitor be added to the RNA prior to storage at -70°C. |
| • |
RNA
Size Limits: From 200 bases
up to 35 kb. |
| • |
Sample
Sources: Whole blood (fresh or stored in ZR
RNA Buffer™). |
|
| DESCRIPTION
The ZR Whole Blood Total RNA Kit™
provides a hassle-free method for the rapid isolation of
total RNA from whole-blood in as little as 15 minutes. This
simple procedure is based on the use of a unique single
buffer system combined with Fast-Spin column technology.
The procedure is easy: just add the buffer to a blood sample
and the extracted RNA is then isolated in RNase-free water
using a Zymo-Spin IIIC™ Column. Optionally, the RNA
can be further purified and concentrated using the provided
Zymo-Spin IC™ Column. RNA can be isolated immediately
from fresh samples or at a later time from blood stored
in ZR RNA Buffer™. This product is designed to purify
and concentrate RNA from blood for subsequent RNA based
methods including RT-PCR, hybridization, etc. |
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ORDERING INFORMATION:
| Products |
Protocol |
Cat
No. |
Size |
Price |
|
ZR
Whole-Blood Total RNA Kit™
Reagents provided are for 50 preps
|
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R1020 |
1
kit |
$198.00 |
|
ZR
Whole-Blood Total RNA Kit™
Reagents provided are for 100 preps
|
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R1021 |
1
kit |
$355.00 |
|
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Components
Available Separately |
| ZR
RNA Buffer |
R1020-1-50 |
50
ml |
$68.00 |
|
| R1020-1-100 |
100
ml |
$122.00 |
|
| RNA
Pre-Wash Buffer |
R1020-2-25 |
25
ml |
$20.00 |
|
| R1020-2-50 |
50
ml |
$30.00 |
|
| RNA
Wash Buffer (concentrate) |
R1003-3-2.4 |
2.4
ml |
$9.00 |
|
| R1003-3-6 |
6
ml |
$15.00 |
|
| R1003-3-12 |
12
ml |
$26.00 |
|
| R1003-3-24 |
24
ml |
$52.00 |
|
| R1003-3-48 |
48
ml |
$92.00 |
|
| Dnase/RNase
Free water |
W1001-1 |
1 ml |
$2.00 |
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| W1001-4 |
4
ml |
$3.00 |
|
| W1001-6 |
6
ml |
$4.00 |
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| W1001-10 |
10
ml |
$6.00 |
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| Zymo-Spin
IC™ Columns (capped) |
C1004-50 |
50
columns |
$46.00 |
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| C1004-250 |
250
columns |
$197.00 |
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| Zymo-Spin
IIIC Columns™ (capped) |
C1006-50 |
50
columns |
$42.00 |
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| C1006-250 |
250
columns |
$198.00 |
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