| DESCRIPTION
The “outgrowth” step in yeast transformation
protocols is often critical for increasing overall yeast
transformation efficiency. Following treatment of yeast
with lithium acetate and polyethylene glycol solutions,
it is often necessary to add medium to allow for a period
of outgrowth, which normally results in increased cell survival.
This is useful when attempting to maximize transformation
efficiencies for library screening or when transforming
yeast with multiple plasmids. YPD Plus™ is a special
formulation of conventional YPD medium that increases yeast
transformation efficiencies by > 50% when used for outgrowth.
YPD Plus™ is recommended for mutant
yeast strains exhibiting poor growth characteristics that
are not amenable to transformation. Simply supplement a
yeast transformation reaction mixture with YPD Plus™
to achieve consistent increases in yeast transformation
efficiencies.
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