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Yeast
Lytic Enzyme - Zymolyase™
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Available
as either liquid or lyophilized Zymolyase. |
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Optimized
concentration (5 U/µl) for maximum lytic activity. |
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Free
of DNase/RNase activity. |
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Optimal
Zymolyase activity is observed from about 30°-37°C.
Lytic activity ceases at higher temperatures.
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SPECIFICATIONS
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Essential
enzyme activities: ß-1,3-glucan laminaripentaohydrolase
and b-1,3-glucanse. |
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Protein
contents: Approximately 10-15 mg/ml. |
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Other
contaminants: Trace amount of Protease, ca. 1.5 units
per 10 µl; DNase and RNase: none detectable. |
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Enzyme
lytic activity: Activity is lost in 5 minutes at 60°C. |
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Unit
Definition: One lytic unit is defined as a 10% decrease
in O.D. at 800 nm for 30 minutes. |
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Assay
Condition: 50 mM potassium phosphate, pH 7.5, 10 mM
2-mercaptoethanol in 1 ml yeast cell suspension of an O.D. of
0.8 to 1.0 at 800 nm. |
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Concentration:
5 units (U) per µl. |
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Storage:
Recommended at or below -70°C. |
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Stability:
Zymolyase is stable for over 1 year at -20°C or many years
below -70°C. |
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DESCRIPTION
Digestion of yeast and fungal cell walls is necessary for
many experimental procedures including spheroplasting, immunofluorescence,
transformation, protein purification, and others. The use
of lytic enzymes like Zymolyase is routinely
used for digestion. Zymo Research’s liquid and lyophilized
forms of the yeast lytic enzyme Zymolyase is prepared from
Arthrobacter luteus. The buffer used to solubilize the Zymolyase
is optimized to confer maximal levels of enzymatic activity.
The main enzymatic activities are ß-1, 3 glucanase
and ß-1, 3-glucan laminaripentao-hydrolase, which
hydrolyze glucose polymers at the ß-1,3- glucan linkages
releasing laminaripentaose as the principal product. |
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ORDERING INFORMATION:
| Products |
Protocol |
Cat
No. |
Size |
Price |
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| Lyophilized
Zymolyase |
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E1004 |
1,000
units |
$52.00 |
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E1005 |
2,000
units |
$86.00 |
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| Components
Available Separately |
| R-Zymolyase |
E1006 |
1,000
units (lyophilized) includes 500 µl storage buffer |
$65.00 |
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