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Zymoprep
I™
Yeast
Plasmid Minipreparation Kit
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Simple method for yeast plasmid minipreparation. |
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Highly efficient. |
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No
glass beads, no phenol, no vortexing. |
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Achieves
great results whether you use colony or liquid cultures.
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SPECIFICITY
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Formats: Isopropanol Precipitation (Zymoprep™
I) or Spin Column (Zymoprep™ II). |
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Plasmid
Purity: (Zymoprep™ I) Relatively “crude”
plasmid DNA preparation suitable for transformation, PCR, or
Western blotting. (Zymoprep™ II) Relatively pure plasmid
DNA preparation suitable for transformation, PCR, electroporation,
or Western blotting, |
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Recovery
Volume: 35 µl. |
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DNA
Size: Plasmid DNA up to 23 kb. |
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DESCRIPTION
Zymoprep™ is the new, simple
and efficient yeast plasmid miniprep that is based on the
old E. coli alkaline lysis method with our Zymolyase
added in the first solution. There is no need for glass
beads, phenol, or vortexing. Reliably recover your plasmid
from yeast cells every time whether you use colonies, patches
on plates, or liquid cultures. The total plasmid yield is
0.01-0.3 ng for most 2µ based plasmids from 1.5 ml
overnight cultures. This kit also works well with low-copy
number yeast plasmids. The recovered plasmid is in TE buffer
and can be used for E. coli transformations, Western
blots, PCR, etc. |
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ORDERING INFORMATION:
| Products |
Protocol |
Cat
No. |
Size |
Price |
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Zymoprep™
Yeast Plasmid Miniprep I
Reagents provided are for 100 preps.
(Isopropanol precipitation method)
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D2001 |
1
set |
$79.00 |
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| Components
Available Separately |
| Lyophilized
Zymolyase (includes 500 µl of Storage Buffer) |
E1004 |
1,000
units |
$52.00 |
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| E1005 |
2,000
units |
$86.00 |
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