About 5-hmC Glucosyltransferase
5-hmC Glucosyltransferase from Zymo Research is a highly active enzyme that specifically tags 5-hydroxymethylcytosine in DNA with a glucose moiety yielding glucosyl-5-hydroxymethylcytosine (Figure 1).
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Figure 1: 5-hmC Glucosyltransferase transfers a glucose moeity from uridine diphosphoglucose (UDPG) onto preexisting 5-hydroxymethylcytosines within DNA.
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Glucosylation of 5-hydroxymethylcytosine by 5-hmC Glucosyltransferase can be used for sequence specific and locus specific (see Cat. Nos. D5410 & D5411), as well as global quantification of 5-hydroxymethylcytosine.
Specifications
| Applications | • Used in method for sequence and locus specfic detection of 5-hydroxymethylcytosine within DNA • Global quantification of 5-hydroxymethylcytosine | ||||||||||||||
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| Storage | 5-hmC Glucosyltransferase is guaranteed for 12 months at -20°C. Long term storage at -80°C is recommended. Avoid multiple freeze thawing. | ||||||||||||||
| Unit Definition | One unit (1 U) is defined as the amount of enzyme needed to “protect” 1μg of 5hmC DNA Standard [D5405-3] from GlaI digestion via glucosylation in a reaction incubate at 30°C for 1 hour. | ||||||||||||||
| Standard Reaction Setup |
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| Enzyme Concentration | 2 units/µl |
