DNA Degradase™

Quick and simple procedure for completely degrading DNA into its individual nucleotide component for quantitative analysis (e.g., whole-genome methylation analysis by HPLC, TLC, etc.).
1 hour, single-enzyme digest vs. conventional 6 - 16 hour multi-step enzyme digestion protocols.

Product Name Size Catalog # Price Qty
DNA Degradase™ 500 Units E2016
$126.00
DNA Degradase™ 2000 Units E2017
$402.00

About DNA Degradase™

DNA Degradase™ from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleotide components. DNA Degradase™ is ideal for whole-genome DNA methylation analysis by a number of downstream applications (i.e., HPLC, TLC, etc.). Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.

Specifications

Format Provided in solution (10 units/µl) w/ 10X Reaction Buffer
Storage Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage should be ≤ -70°C.
Unit Definition One unit (1 U) is defined as the amount of enzyme required to degrade 1 µg of λ DNA in a total reaction volume of 25 µl for 1 hour at 37°C.
Reaction Conditions DNA Degradase™ in 1X DNA Degradase™ Reaction Buffer. Incubate reaction mixtures at 37°C for ≥1 hour.
Inactivation Heat-inactivate at 70°C for 20 minutes.
Standard Reaction Setup The setup (below) is an example of a typical DNA Degradase™ reaction in a 25 µl final volume.
2 µl        DNA at 500 ng/µl
2.5 µl     10X DNA Degradase™ Reaction Buffer
1 µl        DNA Degradase™ (10 units/µl)
19.5 µl   ddH2O
25 µl      Total Volume
  
Incubate at 37°C for 2 hours.

 

DNA Degradase™ efficiently degrades DNA: 1 µg, 5 µg, and 10 µg of λ DNA incubated with 1 µl (10 U) of DNA Degradase™ in 25 µl reaction volume and incubated at 37°C for 1 hour.

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