About Zymoclean™ Gel RNA Recovery Kit
The Zymoclean™ Gel RNA Recovery Kit provides a quick and efficient purification method for recovery of RNA fragments from agarose gels. The procedure combines a unique, single-step agarose dissolving/RNA binding buffer with Fast-Spin column technology to yield high quality, purified RNA in just minutes. The purified RNA is eluted into small volumes of DNase/RNase-free water for highly concentrated samples suitable for subsequent RNA-based manipulations. Compatible with MOPS, TAE, and TBE buffered agarose gels (formaldehyde up to 2.0%).
Specifications
| Format | Spin Column |
|---|---|
| Processing Time | 30 min |
| Sample Sources | Single- or double-stranded RNA fragments (≥200 nucleotides) resolved in MOPS, TAE and TBE buffered agarose gels. |
| RNA Purity | High quality RNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations. |
| RNA Recovery | The recovery rate for fragments ≥500 nucleotides is ≥80 %. Total binding capacity of the supplied Zymo-Spin IC™ Columns is ≥5 µg. |
| RNA Storage | RNA is eluted with RNase-free water and can be stored at ≤ -70°C. The addition of RNase inhibitors is optional but highly recommended for prolonged storage. |
| Equipment Needed | Microcentrifuge, 37 to 65ºC heat source. |
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The recovery of RNA from an agarose gel. Different sized RNAs on the left were excised from the gel and recovered using the Zymoclean™ Gel RNA Recovery Kit (lanes 1-4)
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