About ssDNA/RNA Clean & Concentrator™
The ssDNA/RNA Clean & Concentrator™ provides a simple and reliable method for the rapid separation, clean-up, and concentration of up to ~5 µg (per prep.) of single stranded DNA and/or RNA from double stranded species (e.g., genomic DNA). This simple 10 minutes procedure is based on the use of a unique single-buffer system and Fast-Spin column technology. Single stranded DNA or RNA ≥ 17 to 200 nucleotides (e.g., short transcripts, probes, primers) can be safely treated and recovered using this kit. The result is highly concentrated, purified DNA/RNA that is suitable for subsequent molecular methods including PCR, RT-PCR, hybridization, etc.
| Format | Spin Column |
|---|---|
| Processing Time | 10 min |
| Equipment | Microcentrifuge |
| Sample Sources | Mixtures of double and single stranded DNA and RNA species with single stranded fragments 17 to 200 nucleotides (e.g., short transcripts, probes, primers). |
| Elution Volume | ≥ 6µl |
| Storage | ≤-70°C. The addition of RNase inhibitors is highly recommended for prolonged storage. |
| Purity | High quality DNA/RNA (A260/A280 >1.8, A260/A230 >1.8) suitable for all downstream manipulations. |
| Recovery | Up to 5 μg ssDNA/RNA |
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| Step 1 | Add 2 volumes of DNA/RNA Binding Buffer to the sample1 (e.g., 200 μl buffer to 100 μl sample) and mix well. |
|---|---|
| Step 2 | Transfer the mixture to the Zymo-Spin™ IIC Column in a Collection Tube and centrifuge at ≥12,000 x g for 1 minute. Save the flow-through! |
| Step 3 | Add 1 volume ethanol (95-100%) to the flow-through in the Collection Tube (e.g., 300 μl ethanol to 300 μl flow-through), and mix well by pipetting. |
| Step 4 | Transfer the mixture to the Zymo-Spin™ IC Column in a Collection Tube and centrifuge at ≥12,000 x g for 1 minute. Discard the flow-through. |
| Step 5 | Add 400 µl DNA/RNA Prep Buffer to the column and centrifuge at ≥12,000 x g for 1 minute. Discard the flow-through. |
| Step 6 | Add 700 µl DNA/RNA Wash Buffer to the column and centrifuge at ≥12,000 x g for 30 seconds. Discard the flow-through and repeat Step 6 with 400 µl DNA/RNA Wash Buffer. |
| Step 7 | Centrifuge the Zymo-Spin™ IC Column in an emptied Collection Tube at ≥12,000 x g for 2 minutes. Transfer the column into an RNase-Free Tube |
| Step 8 | Add ≥6 µl DNase/RNase-Free Water2 directly to the column matrix and let stand for 1 minute at room temperature. Centrifuge at 10,000 x g for 30 seconds. The eluted DNA/RNA can be used immediately or stored at or below -20°C. |
