About ZR-Duet™ DNA/RNA MiniPrep
The ZR-Duet™ DNA/RNA MiniPrep provides a quick method for parallel purification of high quality genomic DNA and total RNA from small amounts of cells and tissue. The kit isolates both genomic DNA and large and small RNA species without the use of phenol or reducing agents. Small RNAs (e.g., tRNAs, microRNAs) can be recovered following a simple adjustment of the RNA isolation protocol "“ no extra steps are required! Both DNA and RNA (up to ~25 μg each) from 102 to 107 cells can be isolated in less than 15 minutes.
Specifications
| Format | Spin Column |
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| DNA Recovery | Up to 25 μg DNA/RNA can be eluted into ≥ 50 μl. |
| Processing Time | 15 min |
| Equipment | Microcentrifuge |
| DNA Size Limits | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. Total RNA ≥ 17 nucleotides can be recovered. |
| Sample Sources | Cells, small amounts of easy-to-lyse tissue, buffy coat, buccal cells, plasma, serum, and other biological liquids. Not compatible with whole blood. (For purification of DNA from whole blood see the Quick-gDNA™ MiniPrep (D3024) and for RNA see the ZR Whole-Blood RNA MiniPrep (R1020)) |
| DNA Purity | High quality genomic DNA and total RNA (A260/A280 > 1.8, A260/A230 > 1.8) is recovered. |
| Storage | -70°C |
| RNA Purity | High quality genomic DNA and total RNA (A260/A280 > 1.8, A260/A230 > 1.8) is recovered. |
| RNA Recovery | Up to 25 μg DNA/RNA can be eluted into ≥ 25 μl |
| Sample Size | 102 to 5x106 cells in suspension or as tissue. |
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DNA and RNA purified using the ZR-Duet™ DNA/RNA MiniPrep. Genomic DNA (lane 1, 2) and total RNA (lane 3, 4) isolated from human epithelial cells (HCT 116) with the ZR-Duet™ DNA/RNA MiniPrep. M is a 1 kb DNA Marker (Zymo Research).
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PCR amplification of ß-actin transcript (353 bp fragment shown) following DNA and RNA isolation from human epithelial cells (HCT 116) with the ZR-Duet™ DNA/RNA MiniPrep: PCR positive control (DNA template; lane 1, 2, 3), PCR negative control (RNA template; lane 4, 5, 6), RT-PCR (lane 7, 8, 9). M1 and M2 are 1 kb and 100 bp DNA Markers, respectively (Zymo Research).
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