About ZR Viral DNA/RNA Kit™
The ZR Viral DNA/RNA Kit™ provide for rapid, single column isolation of high-quality viral nucleic acids from a wide range of biological sources. The kit can be used to successfully isolate viral DNA and RNA from cell-free body fluids as well as cellular suspensions at concentrations ≤ 1 x 105 cells/ml. The procedure employs a single buffer system that facilitates viral particle lysis and allows for the subsequent DNA/RNA binding onto the matrix of the Zymo-Spin™ IC Column. The nucleic acids are washed then eluted with DNase/RNase-free Water. The eluted DNA and RNA are suitable for use in various subsequent procedures including RT/PCR.
Specifications
| Format | Spin Column |
|---|---|
| DNA Recovery | Up to 10 μg total DNA and/or RNA per column can be eluted with ≥ 10 µl water. |
| Processing Time | 5 min |
| Equipment | Microcentrifuge |
| DNA Size Limits | Capable of purifying small DNA/RNA fragments > 50 bases and large sized DNA/RNA > 200 kb. |
| Sample Sources | Cells, serum, plasma, culture supernatants. |
| DNA Purity | RT/PCR quality DNA and RNA (A260/A280 > 1.8, A260/A230 > 1.8) is recovered. |
| Storage | Eluted DNA/RNA can be stored at ≤ -70°C |
| RNA Purity | RT/PCR quality DNA and RNA (A260/A280 > 1.8, A260/A230 > 1.8) is recovered. |
| RNA Recovery | Up to 10 μg total DNA and/or RNA per column can be eluted with ≥ 10 µl water. |
| Sample Size | ≤ 200 µl (e.g. plasma, serum) can be processed per standard preparation. |
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Viral nucleic acids were isolated from liquid samples using the ZR Viral DNA/RNA Kit™. Isolated viral nucleic acids were reverse transcribed/amplified using a coupled RT-real-time PCR system (Zymo Research). Ct values for measles, influenza type A (FluA), and herpes-simplex (HSV) viruses were 23.05 (diamonds), 24.56 (triangles), 22.92 (circles), respectively. Negative control "“ RT-PCR (no template w/ HSV specific primers). Positive control "“ PCR (HSV template w/ HSV primers).
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