About ZR Plant RNA MiniPrep™
The ZR Plant RNA MiniPrep™ provides for rapid isolation of total RNA from various plant samples (e.g., leaves, stems, buds, flowers, fruit, seeds, etc.). The kit allows for high-capacity DNA elimination and efficient purification of total RNA. The RNA is eluted into volumes as little as 25 µl. For polyphenolic inhibitor removal, the eluted RNA is filtered using Zymo-Spin™ IV-HRC spin devices. The RNA is suitable for use in various downstream procedures including RT-PCR. The entire RNA isolation procedure typically takes about 15 minutes.
Specifications
| Format | Spin Column |
|---|---|
| Processing Time | 15 min |
| Equipment | Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional). |
| Sample Sources | Leaves, stems, buds, flowers, fruit, seeds, etc. |
| RNA Purity | High quality RNA (A260/A280>1.8, A260/A230>1.8) recovered. |
| RNA Recovery | Up to 25 µg RNA can be eluted into ≥ 25 µl, allowing for a highly concentrated sample. |
| RNA Storage | RNA is eluted with RNase-free water and can be stored at ≤ -70°C. The addition of RNase inhibitors is optional but highly recommended for prolonged storage. |
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Isolation of total RNA from 10 mg of a fresh leaf material (Nicotiana sp.) using the ZR Plant RNA MiniPrep™. Leaves were minced then processed using a FastPrep®-24 instrument (MP Biomedicals). Samples 1 and 2 were loaded in 2x and 1x volume aliquots, respectively, and resolved in a 1% (w/v) nondenaturing agarose gel. RNA Millenium™ Markers (Ambion) were used as size standards
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