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ZR-96 Quick-RNA™

High throughput (96-well) isolation of total RNA (including small RNAs) from a wide range of samples - single to 106 cells.
DNA-free RNA for use in any downstream application. DNase I included.

Product Size Catalog # Price Qty
ZR-96 Quick-RNA™ 2x96 Preps R1052
ZR-96 Quick-RNA™ 4x96 Preps R1053

About ZR-96 Quick-RNA™

The ZR-96 Quick-RNA kit is an innovative product designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 106) and tissue samples (up to 5 mg).  The procedure combines a unique buffer system with Fast-Spin plate technology to yield high quality total RNA (including small RNAs ~17-200 nt) in about 30 minutes.

The procedure is simple:  Add the provided RNA Lysis Buffer to a sample, then purify the RNA using the provided Silicon-A Plate.  The result is highly-concentrated, DNA-free RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, sequencing etc.

Sample Sources Cells or tissue samples, yeast, plant, bacteria, buccal cells, buffy coat, plasma, serum, and other biological liquids. Compatible with DNA/RNA Shield™ and RNAlater™.
Storage Samples homogenized in RNA Lysis Buffer are stable and can be stored frozen prior to purification.
RNA Purity High quality RNA (A260/A280 >1.8, A260/A230 >1.8) suitable for all downstream RNA-based manipulations.
RNA Recovery Up to 10 µg RNA can be eluted into ≥25 µl RNase-free water allowing for a highly concentrated sample.
Sample Size Up to 106 cells or 5 mg tissue.
RNA Storage RNA is eluted with RNase-free water and can be stored frozen. RNase inhibitors can be included for prolonged storage.
Equipment Needed Centrifuge/rotor compatible with 96-well plates.

The ZR-96 Quick-RNA™ yields high quality total RNA. High levels of genomic DNA contamination are present in the preps from Suppliers Q & P but not with the ZR-96 Quick-RNA™. Total RNA was isolated from human epithelial cells (sans DNase treatment).

RNA isolated with the ZR-96 Quick-RNA™ is DNA-free. Samples isolated with Supplier Q's kit are provided for comparison. Total RNA was isolated from 106 human epithelial cells (with in-column DNase treatments for both kits). Each amplification curve represents an average of three independent isolation experiments.

Step 1 Go here for the updated protocol
For specific notes and additional information, please see the product protocol PDF.
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