About Quick-DNA/RNA™ FFPE Kit
The Quick-DNA/RNA™ FFPE Kit provides a simple and reliable method for total nucleic acid isolation (DNA & RNA) from the same formalin-fixed, paraffin embedded (FFPE) tissue sample. The unique chemistries of the product have been optimized for maximum recovery of DNA and of both large and small RNA species. Simply deparaffinize tissues using Deparaffinization Solution, digest using Proteinase K, heat to reverse chemical crosslinks, and then purify using Zymo-Spin® column technology. The result is high-quality DNA & total RNA (including small RNAs 17-200 nt) that is DNA-free and is ready for RT-PCR, hybridization, sequencing, etc.
|Equipment||Microcentrifuge, vortex, and heat block.|
|Sample Sources||Up to 25 mg tissue from paraffin block or up to four (4) tissue sections (≤20 µm thick) with a total surface area ~20 cm2. It is recommended to use 1-2 sections if performing the protocol for the first time. Compatible with fresh/frozen tissue specimens.|
|Storage||DNA and RNA eluted with DNase/RNase-Free Water can be stored at ≤70ºC. The addition of RNase inhibitors in highly recommended for prolonged storage.|
|Purity||High quality DNA and RNA (A260/A280 >1.8, A260/A230 >1.8) are recovered.|
|Size Limit||Capable of recovering DNA and total RNA ≥17 nucleotides.|
|Recovery||The DNA/RNA binding capacity of the Zymo-Spin™ IIC Column is ~50 µg.|