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High performance sample prep technology for total nucleic acid (DNA & RNA) recovery from the same FFPE tissue sample/section.
High quality DNA and DNA-free RNA is ready for use in any downstream application. DNase I included.

Product Size Catalog # Price Qty
Quick-DNA/RNA™ FFPE Kit 50 Preps R1009

About Quick-DNA/RNA™ FFPE Kit

The Quick-DNA/RNA FFPE Kit provides a simple and reliable method for total nucleic acid isolation (DNA & RNA) from the same formalin-fixed, paraffin embedded (FFPE) tissue sample. The unique chemistries of the product have been optimized for maximum recovery of DNA and of both large and small RNA species. Simply deparaffinize tissues using Deparaffinization Solution, digest using Proteinase K, heat to reverse chemical crosslinks, and then purify using Zymo-Spin® column technology. The result is high-quality DNA & total RNA (including small RNAs 17-200 nt) that is DNA-free­ and is ready for RT-PCR, hybridization, sequencing, etc.  

Equipment Microcentrifuge, vortex, and heat block.
Sample Sources Up to 25 mg tissue from paraffin block or up to four (4) tissue sections (≤20 µm thick) with a total surface area ~20 mm2. It is recommended to use 1-2 sections if performing the protocol for the first time. Compatible with fresh/frozen tissue specimens.
Storage DNA and RNA eluted with DNase/RNase-Free Water can be stored at ≤ -70ºC. The addition of RNase inhibitors in highly recommended for prolonged storage.
Purity High quality DNA and RNA (A260/A280 >1.8, A260/A230 >1.8) are recovered.
Size Limit Capable of recovering DNA and total RNA ≥17 nucleotides.
Recovery The DNA/RNA binding capacity of the Zymo-Spin™ IIC Column is ~50 µg.

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