About ZR-96 RNA Clean & Concentrator™
The ZR-96 RNA Clean & Concentrator™ provides a simple and reliable method for high throughput (96-well) purification and concentration of up to ~25 µg/well of high-quality RT-PCR-ready RNA. The procedure is based on the use of a unique single-buffer system and Zymo-Spin™ plate technology.
The kit allows for efficient RNA clean-up from up to 96 samples with the supplied Zymo-Spin™ I-96 Plate. The RNA is washed twice then eluted and concentrated into ≥10 µl of DNase/RNase-free water. Protocol for DNase I digestion is provided.
RNA ≥17 nucleotides can be safely treated and recovered using this kit. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.
The entire procedure typically takes about 30 minutes.
|Sample Sources||DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol®/chloroform or similar extraction.|
|RNA Purity||High quality RNA (A260/A280 >1.8, A260/A230 >1.8) suitable for reverse transcription, microarray, sequencing etc.|
|RNA Recovery||Up to ~25 µg RNA/well can be eluted into as little as ≥10 µl RNase-free water allowing for a highly concentrated sample.|
|RNA Storage||RNA is eluted with RNase-free water and can be stored at ≤-70 ºC. The addition of RNase inhibitors is optional but highly recommended for prolonged storage.|
|Equipment Needed||Centrifuge with 96-well plate carrier.|
|RNA Size Limits||From 17 nt to ~23 kb.|
|Click Here to download the protocol|